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A naturally occurring 46-amino acid deletion of cytidine monophospho-N-acetylneuraminic acid hydroxylase leads to a change in the intracellular distribution of the protein

  • Papers Dedicated To Dr Sen-Itiroh Hakomori
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Abstract

Cytidine monophospho-N-acetylneuraminic acid (CMP-NeuAc) hydroxylase is a key enzyme for the expression ofN-glycolylneuraminic acid. The molecular cloning of this enzyme from mouse liver has been described in our previous report (Kawano T, Koyama S, Takematsu H, Kozutsumi Y, Kawasaki H, Kawashima S, Kawasaki T, Suzuki A (1995)J Biol Chem 270: 16458–63). During the cDNA cloning, a cDNA containing a truncated open reading frame (ORF) was isolated. This clone encodes a protein of 531 amino acids which lacks 46 amino acids in the middle of the normal full-length protein. The percentage of this mRNA containing the truncated ORF out of the total population of CMP-NeuAc hydroxylase mRNA in various mouse tissues was about 10–25%. The truncated protein was expressed in COS-1 cells, but did not show any enzymatic activity. The truncated protein was localized to the region which appeared to be the endoplasmic reticulum, whereas the full-length protein with normal enzymatic activity was detected in the cytosol. These data suggest that this naturally occurring 46-amino acid deletion leads to a change in the intracellular distribution of CMP-NeuAc hydroxylase, and a loss in the activity of this enzyme.

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Abbreviations

NeuAc:

N-acetylneuraminic acid

NeuGe:

N-glycolylneuraminic acid

ORF:

open reading frame

ER:

endoplasmic reticulum

PCR:

polymerase chain reaction

RT-PCR:

reverse transcription-polymerase chain reaction

TBS:

Tris-buffered saline

SDS-PAGE:

sodium dodecyl sulfate-polyacrylamide gel electrophoresis

pI:

isoelectric point

bp:

base pair(s)

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Authors and Affiliations

  1. Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, 606, Kyoto, Japan

    Susumu Koyama, Toshiyuki Yamaji, Hiromu Takematsu, Yasunori Kozutsumi & Toshisuke Kawasaki

  2. Department of Biochemistry, Institute of Medical Science, University of Tokyo, Shirokanedai, Minato-ku, 108, Tokyo, Japan

    Takehiro Kawano

  3. Department of Membrane Biochemistry, Tokyo Metropolitan Institute of Medical Science, Honkomagome, Bunkyo-ku, 113, Tokyo, Japan

    Akemi Suzuki

Authors
  1. Susumu Koyama
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  2. Toshiyuki Yamaji
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  3. Hiromu Takematsu
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  4. Takehiro Kawano
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  5. Yasunori Kozutsumi
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  6. Akemi Suzuki
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  7. Toshisuke Kawasaki
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Additional information

This paper is dedicated to Dr Sen-ichiro Hakomori in celebration of his 65th birthday.

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Koyama, S., Yamaji, T., Takematsu, H. et al. A naturally occurring 46-amino acid deletion of cytidine monophospho-N-acetylneuraminic acid hydroxylase leads to a change in the intracellular distribution of the protein. Glycoconjugate J 13, 353–358 (1996). https://doi.org/10.1007/BF00731467

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  • DOI: https://doi.org/10.1007/BF00731467

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