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Constitutively hyposialylated human T-lymphocyte clones in the Tn-syndrome: binding characteristics of plant and animal lectins

  • Glycoconjugate Journal
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Abstract

Previously, β1,3-galactosyltransferase-deficient (Tn+) and normal (TF+) T-lymphocyte clones have been established from a patient suffering from Tn-syndrome [Thurnheret al. (1992)Eur J Immunol 22: 1835–42], Tn+ T lymphocytes express only Tn antigen (GalNAcα1-O-R) while other O-glycan structures such as sialosyl-Tn (Neu5Acα2,6GalNAcα1-O-R) or TF (Galβ1-3GalNAcα1-O-R) antigens are absent from these cells as shown by flow cytometry using specific mABs for TF and sialosyl-Tn antigen, respectively. Normal T lymphocytes express the TF antigen and derivatives thereof. The surface glycans of Tn+ and TF+ cells were then analysed by flow cytometry using the following sialic acid-binding lectins:Amaranthus caudatus (ACA),Maackia amurensis (MAA),Limax flavus (LFA),Sambucus nigra (SNA) andTriticum vulgare (WGA). Equal and weak binding of MAA and SNA to both TF+ and Tn+ cells was found. WGA, LFA and ACA bound more strongly to TF+ cells than to Tn+ cells. Binding of ACA to TF+ cells was enhanced after sialidase treatment. To investigate the possible biological consequences of hyposialylation, binding of three sialic acid-dependent adhesion molecules to Tn+ and TF+ cells was estimated using radiolabelled Fc-chimeras of sialoadhesin (Sn), myelin-associated glycoprotein (MAG) and CD22. Equal and strong binding of human CD22 to both TF+ and Tn+ cells was found. Whereas binding of Sn and MAG to TF+ cells was strong (100%), binding to Tn+ cells amounted only to 33% (Sn) and 19% (MAG). These results indicate that thein vivo interactions of T lymphocytes in the Tn syndrome with CD22 are not likely to be affected, whereas adhesion mediated by Sn or MAG could be strongly reduced.

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Authors and Affiliations

  1. Instit ute of Physiology, University of Zürich, Winterthurerstrasse 190, CH-8057, Zürich, Switzerland

    Kristina Mrkoci & Eric G. Berger

  2. Biochemisches Institut, University of Kiel, 24098, Kiel, Germany

    Sørge Kelm & Roland Schauer

  3. Imperial Cancer Research Fund Laboratories, Institute of Molecular Medicine, University of Oxford, OX3 9DU, Oxford, UK

    Paul R. Crocker

Authors
  1. Kristina Mrkoci
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  2. Sørge Kelm
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  3. Paul R. Crocker
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  4. Roland Schauer
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  5. Eric G. Berger
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Mrkoci, K., Kelm, S., Crocker, P.R. et al. Constitutively hyposialylated human T-lymphocyte clones in the Tn-syndrome: binding characteristics of plant and animal lectins. Glycoconjugate J 13, 567–573 (1996). https://doi.org/10.1007/BF00731444

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  • DOI: https://doi.org/10.1007/BF00731444

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