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Glycoconjugate Journal

, Volume 13, Issue 4, pp 555–566 | Cite as

Structures and contribution to the antigenicity of oligosaccharides of Japanese cedar (Cryptomeria japonica) pollen allergenCry j I: relationship between the structures and antigenic epitopes of plantN-linked complex-type glycans

  • Haruko Ogawa
  • Ako Hijikata
  • Maho Amano
  • Kyoko Kojima
  • Hisako Fukushima
  • Ineo Ishizuka
  • Yoshie Kurihara
  • Isamu Matsumoto
Glycoconjugate Journal

Abstract

The oligosaccharide structures ofCry j I, a major allergenic glycoprotein ofCryptomeria japonica (Japanese cedar, sugi), were analysed by 400 MHz1H-NMR and two-dimensional sugar mapping analyses. The four major fractions comprised a series of biantennary complex type N-linked oligosaccharides that share a fucose/xylose-containing core and glucosamine branches including a novel structure with a nongalactosylated fucosylglucosamine branch.

Rabbit polyclonal anti-Cry j I IgG antibodies cross-reacted with three different plant glycoproteins having the same or shorter N-linked oligosaccharides asCry j I. ELISA and ELISA inhibition studies with intact glycoproteins, glycopeptides and peptides indicated that both anti-Cry j I IgGs and anti-Sophora japonica bark lectin II (B-SJA-II) IgGs included oligosaccharide-specific antibodies with different specificities, and that the epitopic structures against anti-Cry j I IgGs include a branch containing α1–6 linked fucose and a core containing fucose/xylose, while those against anti-B-SJA-II IgGs include nonreducing terminal mannose residues. The cross-reactivities of human allergic sera to miraculin andClerodendron Trichotomum lectin (CTA) were low, and inhibition studies suggested that the oligosaccharides onCry j I contribute little or only conformationally to the reactivity of specific IgE antibodies.

Keywords

Cry jpollen allergen carbohydrate epitope N-linked oligosaccharide 

Abbreviations

Cry j I

a major allergenic glycoprotein ofCryptomeria japonica

B-SJA-II

Sophora japonica bark lectin II

CTA

Clerodendron trichotomum lectin

TFMS

trifluoromethanesulfonic acid

HRP

horseradish peroxidase

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Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • Haruko Ogawa
    • 1
  • Ako Hijikata
    • 1
  • Maho Amano
    • 1
  • Kyoko Kojima
    • 1
  • Hisako Fukushima
    • 1
  • Ineo Ishizuka
    • 2
  • Yoshie Kurihara
    • 3
  • Isamu Matsumoto
    • 1
  1. 1.Department of Chemistry, Faculty of ScienceOchanomizu UniversityTokyoJapan
  2. 2.Department of Biochemistry, School of MedicineTeikyo UniversityTokyoJapan
  3. 3.Department of Chemistry, Faculty of EducationYokohama National UniversityYokohamaJapan

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