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An antibody-lectin sandwich assay for the determination of CA125 antigen in ovarian cancer patients

  • Glycosylation And Disease Papers
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Abstract

A two-step forward sandwich assay was developed for the determination of the ovarian tumour associated glycoconjugate antigen CA125 with anti-CA125 Monoclonal antibody B27.1 on the solid phase and125I-labelled wheat germ lectin as tracer in the solution phase. This Mab-lectin heterosandwich assay was optimized and the clinical utility was evaluated in sera from healthy volunteers and ovarian cancer patients. A correlation was established between Mab-lectin assay and the dual monoclonal antibody sandwich assay, TRUQUANT®OV2 RIA, that uses the same MAb B27.1 on the solid phase and a second125I-labelled B43.13 MAb in the solution phase. A potentially improved clinical utility is suggested for the Mab-lectin assay. The unique format seems to identify novel isoforms of CA125 with different carbohydrate side chains that would otherwise be undetectable in the MAb-MAb sandwich assay wherein the paratopes are likely directed to protein determinants.

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Authors and Affiliations

  1. Biomira Research Inc., T6N 2N8, Edmonton, Alberta, Canada

    R. Madiyalakan, M. Kuzma & A. A. Noujaim

  2. Faculty of Pharmacy, University of Alberta, T6N 2N8, Edmonton, Alberta, Canada

    M. R. Suresh

Authors
  1. R. Madiyalakan
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  2. M. Kuzma
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  3. A. A. Noujaim
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  4. M. R. Suresh
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Madiyalakan, R., Kuzma, M., Noujaim, A.A. et al. An antibody-lectin sandwich assay for the determination of CA125 antigen in ovarian cancer patients. Glycoconjugate J 13, 513–517 (1996). https://doi.org/10.1007/BF00731437

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  • DOI: https://doi.org/10.1007/BF00731437

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