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Negative-ion fast atom bombardment tandem mass spectrometry for characterization of sulfated unsaturated disaccharides from heparin and heparan sulfate

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Abstract

Negative-ion fast atom bombardment tandem mass spectrometry has been used in the characterization of non-, mono-, di- and trisulfated disaccharides from heparin and heparan sulfate. The positional isomers of the sulfate group of monosulfated disaccharides were distinguished from each other by negative-ion fast atom bombardment tandem mass spectra, which provide an easy way of identifying the positional isomers. This fast atom bombardment collision induced dissociation mass spectrometry/mass spectrometry technique was also applied successfully to the characterization of di- and trisulfated disaccharides.

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Abbreviations

FABMS:

fast atom bombardment mass spectrometry

CID:

collision induced dissociation

MIKE:

mass analysed ion kinetic energy

MS/MS:

mass spectrometry/mass spectrometry

HPLC:

high performance liquid chromatography

ΔUA:

d-gluco-4-enepyranosyluronic acid

CS:

chondroitin sulfate

DS:

dermatan sulfate

HA:

hyaluronan

Hep:

heparin

HS:

heparan sulfate

ΔUA(1→4) GlcNAc:

2-acetamido-2-deoxy-4-O-(β-d-gluco-4-enepyranosyluronic acid)-d-glucose

ΔUA(1→4)GlcNAc6S:

2-acetamido-2-deoxy-4-O-(β-d-gluco-4-enepyranosyluronic acid)-6-O-sulfo-d-glucose

ΔUA2S(1→4)GlcNAc:

2-acetamido-2-deoxy-4-O-(2-O-sulfo-β-d-gluco-4-enepyranosyluronic acid)-d-glucose

ΔUA2S(1→4)GlcNAc6S:

2-acetamido-2-deoxy-4-O-(2-O-sulfo-β-d-gluco-4-enepyranosyluronic acid)-6-O-sulfo-d-glucose

ΔUA(1→4)GlcN6S:

2-amino-2-deoxy-4-O-(β-d-gluco-4-enepyranosyluronic acid)-6-O-sulfo-d-glucose

ΔUA2S(1→4)GlcN:

2-amino-2-deoxy-4-O-(2-O-sulfo-β-d-gluco-4-enepyranosyluronic acid)-d-glucose

ΔUA2S(1→4)GlcN6S:

2-amino-2-deoxy-4-O-(2-O-sulfo-β-d-gluco-4-enepyranosyluronic acid)-6-O-sulfo-d-glucose

ΔUA(1→4)GlcNS:

2-deoxy-2-sulfamino-4-O-(β-d-gluco-4-enepyranosyluronic acid)-d-glucose

ΔUA(1→4)GlcNS6S:

2-deoxy-2-sulfamino-4-O-(β-d-gluco-4-enepyranosyluronic acid)-6-O-sulfo-d-glucose

ΔUA2S(1→4)GlcNS:

2-deoxy-2-sulfamino-4-O-(2-O-sulfo-β-d-gluco-4-enepyranosyluronic acid)-d-glucose

ΔUA2S(1→4)GlcNS6S:

2-deoxy-2-sulfamino-4-O-(2-O-sulfo-β-d-gluco-4-enepyranosyluronic acid)-6-O-sulfo-d-glucose

ΔUA(1→3)GalNAc:

2-acetamido-2-deoxy-3-O-(β-d-Gluco-4-enepyranosyluronic acid)-d-galatose

ΔUA(1→3)GalNAc4S:

2-acetamido-2-deoxy-3-O-(β-d-gluco-4-enepyranosyluronic acid)-4-O-sulfo-d-galactose

ΔUA(1→3)GalNAc6S:

2-acetamido-2-deoxy-3-O-(β-d-gluco-4-enepyranosyluronic acid)-6-O-sulfo-d-galactose

ΔUA2S(1→3)GalNAc:

2-acetamido-2-deoxy-3-O-(2-O-sulfo-β-d-gluco-4-enepyranosyluronic acid)-d-galactose

ΔUA2S(1→3)GalNAc4S:

2-acetamido-2-deoxy-3-O-(2-O-sulfo-β-d-gluco-4-enepyranosyluronic acid)-4-O-sulfo-d-galactose

ΔUA2S(1→3)GalNAc6S:

2-acetamido-2-deoxy-3-O-(2-O-sulfo-β-d-gluco-4-enepyranosyluronic acid)-6-O-sulfo-d-galactose

ΔUA(1→3)GalNAcDiS:

2-acetamido-2-deoxy-3-O-(β-d-gluco-4-enepyranosyluronic acid)-4,6-di-O-sulfo-d-galactose

ΔUA(1→3)GlcNAc:

2-acetamido-2-deoxy-3-O-(β-d-gluco-4-enepyranosyluronic acid)-d-glucose.

References

  1. Poole AR (1986)Biochem J 236:1–14.

    Google Scholar 

  2. Rouslahti E (1989)J Biol Chem 264:13369–72.

    Google Scholar 

  3. Fransson LÅ (1987)Trends Biochem Sci 12:406–11.

    Google Scholar 

  4. Suzuki S, Mizutani A, Koike Y, Kato M, Yoshida K, Kimata K (1991)Pure Appl Chem 63:545–54.

    Google Scholar 

  5. Al-Hakim A, Linhardt RJ (1991)Anal Biochem 195:68–73.

    Google Scholar 

  6. Yoshida K, Miyauchi S, Kikuchi H, Tawada A, Tokuyasu K (1989)Anal Biochem 177:327–32.

    Google Scholar 

  7. Yamada S, Yoshida K, Sugiura M, Sugahara K (1992)J Biochem (Tokyo) 112:440–47.

    Google Scholar 

  8. Ampofo SA, Wang HM, Linhardt RJ (1991)Anal Biochem 199:3249–55.

    Google Scholar 

  9. Desai UR, Wang HM, Ampofo SA, Linhardt RJ (1993)Anal Biochem 213:120–27.

    Google Scholar 

  10. Carr SA, Reinhold VN (1984)Carbohydr Chem 3:381–401.

    Google Scholar 

  11. Reinhold VN, Carr SA, Green BN, Petitou M, Choay J, Sinay P (1987)Carhohydr Res 161:305–13.

    Google Scholar 

  12. Mallis LM, Wang HM, Loganathan D, Linhardt RJ (1989)Anal Chem 61:1453–58.

    Google Scholar 

  13. Linhardt RJ, Wang HM, Loganathan D, Lamb DJ, Mallis LM (1992)Carbohydr Res 225:137–45.

    Google Scholar 

  14. Lamb DJ, Wang HM, Mallis LM, Linhardt RJ (1992)J Am Soc Mass Spectrom 3:797–803.

    Google Scholar 

  15. Ii T, Okuda S, Hirano T, Ohashi M (1994)Glycoconjugate J 11:123–32.

    Google Scholar 

  16. Nores GA, Hanai N, Levery SB, Eaton HL, Salyan MEK, Hakomori S (1988)Carbohydr Res 179:393–410.

    Google Scholar 

  17. Morita M, Matsubara T, Hayashi A (1990)Proc Japanese Soc Biomed Mass Spectrom (Japan) 15:103–6.

    Google Scholar 

  18. Domon B, Costello CE (1988)Glycoconjugate J 5:397–409.

    Google Scholar 

  19. Garozzo D, Giuffrida M, Impallomeri G, Ballistreri A, Montaudo G (1990)Anal Chem 62:279–86.

    Google Scholar 

  20. Chai W, Green BM, Lawson AM (1993)Proceedings of the 41st ASMS Conference on Mass Spectrometry and Allied Topics 41:85a–85b.

  21. Da Col R, Silvestro L, Naggi A, Torri G, Baiocchi C, Moltrasio D, Cedro A, Viano I (1993)J Chromatogr 647:289–300.

    Google Scholar 

Download references

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Authors and Affiliations

  1. Research and Development Laboratories, Soda Aromatic Co., Ltd., Noda-shi, 270-02, Chiba, Japan

    Tadashi II

  2. Department of Applied Physics and Chemistry, The University of Electro-Communications, Chofu, 182, Tokyo, Japan

    Masayuki Kubota, Takashi Hirano & Mamoru Ohashi

  3. School of Agricultural Science, Nagoya University, Chikusa-ku, 464-01, Nagoya, Japan

    Satoshi Okuda

Authors
  1. Tadashi II
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  2. Masayuki Kubota
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  3. Satoshi Okuda
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  4. Takashi Hirano
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  5. Mamoru Ohashi
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II, T., Kubota, M., Okuda, S. et al. Negative-ion fast atom bombardment tandem mass spectrometry for characterization of sulfated unsaturated disaccharides from heparin and heparan sulfate. Glycoconjugate J 12, 162–172 (1995). https://doi.org/10.1007/BF00731361

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  • DOI: https://doi.org/10.1007/BF00731361

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