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Sialidase of swine influenza A viruses: variation of the recognition specificities for sialyl linkages and for the molecular species of sialic acid with the year of isolation

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Abstract

The sialidase of swine influenza A viruses of N1 and N2 subtypes, isolated from 1930 to 1992, was studied for substrate specificity with ganglio-series, lacto-series type II and GM3 gangliosides containing Neu5Acα2-3Gal, Neu5Gcα2-3Gal and Neu5Acα2-6Gal linkages. All viral sialidases tested showed that the activity for hydrolysing substrates with Neu5Acα2-3Gal was higher than the activities with Neu5Gcα2-3Gal and Neu5Acα2-6Gal linkages. When GM1b, GM3 and sialylparagloboside were used as substrates, the earliest strain (A/Wisconsin/15/30 H1N1, isolated in 1930) showed the activity ratio of Neu5Acα2-6Gal to Neu5Acα2-3Gal to be 0.13:0.2, and the ratio Neu5Gcα2-3Gal/Neu5Acα2-3Gal to be 0.19:0.37, while those strains isolated from 1978 to 1992 exhibited ratios of 0.29:0.58 for Neu5Acα2-6Gal/Neu5Acα2-3Gal and 0.51:0.76 for Neu5Gcα2-3Gal/Neu5Acα2-3Gal. The above results indicate that the substrate specificities of sialidases from swine influenza A viruses towards sialyl linkages and the molecular species of sialic acid are related to the year of isolation, i.e. strains isolated after 1978 exhibited higher activity towards Neu5Acα2-6Gal and Neu5Gcα2-3Gal linkages when compared with strains isolated in an earlier year, 1930.

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Abbreviations

Neu5Ac:

5-N-acetylneuraminic acid

Neu5Gc:

5-N-glycolyneuraminic acid

Gal:

d-galactose

Glc:

d-glucose

Cer:

Ceramide

II3(Neu5Ac)Lac:

Neu5Acα2-3Galβ1-4Glc

GM3(Neu5Acα2-3Gal):

Neu5Acα2-3Galβ1-4Glcβ1-Cer

GM3(Neu5Gcα2-3Gal):

Neu5Gcα2-3Galβ1-4Glcβ1-Cer

GM1b(Neu5Acα2-3Gal):

Neu5Acα2-3Galβ1-3GalNacβ1-4Galβ1-4Glcβ1-Cer

GMlb(Neu5Gcα2-3Gal):

Neu5Gcα2-3Galβ1-3GalNAcβ1-4Galβ1-4Glcβ1-Cer

IV3(Neu5Ac)nLc4Cer:

Neu5Acα2-3Galβ1-3GlcNAcβ1-4Galβ1-4Glcβ1-Cer

IV3(Neu5Gc)nLc4Cer:

Neu5Gcα2-3Galβ1-3GlcNAcβ1-4Galβ1-4Glcβ1-Cer

IV6(Neu5Ac)nLc4Cer:

Neu5Acα2-6Galβ1-3GlcNAcβ1-4Galβ1-4Glcβ1-Cer

TDC:

taurodeoxycholate.

References

  1. Suzuki Y, Matsunaga M, Matsumoto M (1985)J Biol Chem 260:1362–65.

    Google Scholar 

  2. Suzuki Y, Nagao Y, Kato H, Matsumoto M, Nerome K, Nakajima K, Nobusawa E (1986)J Biol Chem 261:17057–61

    Google Scholar 

  3. Suzuki Y, Kato H, Naeve CW, Webster RG (1989)J Virol 63:4298–302.

    Google Scholar 

  4. Suzuki Y, Nakao T, Ito T, Watanabe N, Toda Y, Xu G, Suzuki T, Kobayashi T, Kimura Y, Yamada A, Sugawara K, Nishimura H, Kitame F, Nakamura K, Deya E, Kiso M, Hasegawa A (1992)Virology 189:121–31.

    Google Scholar 

  5. Gottschalk A (1957)Biochim Biophys Acta 23:645–46.

    Google Scholar 

  6. Paulson JC (1985) InThe Receptors, Vol. II (Conn PM ed.) pp. 131–219. Orlando: Academic Press.

    Google Scholar 

  7. Wiley DC, Skehel JJ (1987)Annu Rev Biochem 56:365–94.

    Google Scholar 

  8. Weis W, Brown JH, Cusack S, Paulson JC, Skehel JJ, Wiley DC (1988)Nature 333:426–31.

    Google Scholar 

  9. Kornfeld R, Kornfeld S (1980) InThe Biochemistry of Glycoproteins and Glycolipids (Lennarz WJ ed.) pp. 1–27. New York: Plenum.

    Google Scholar 

  10. Carrol SM, Higa HH, Paulson JC (1981)J Biol Chem 256:8357–63.

    Google Scholar 

  11. Nuss JM, Air GM (1991)Virology 183:496–504.

    Google Scholar 

  12. Baum LG, Paulson JC (1991)Virology 180:10–15.

    Google Scholar 

  13. Paulson JC, Sadler JE, Hill RL (1979)J Biol Chem 254:2120–24.

    Google Scholar 

  14. Cahan LD, Paulson JC (1980)Virology 103:505–9.

    Google Scholar 

  15. Matrosovich MN, Gambaryan AS, Chumakov MP (1992)Virology 188:854–58.

    Google Scholar 

  16. Xu G, Suzuki T, Hanagata G, Deya E, Kiso M, Hasegawa A, Suzuki Y (1993)J Biochem 113:304–7.

    Google Scholar 

  17. Suzuki Y, Suzuki N, Michi H, Matsumoto M (1993)Lipids 20:588–93.

    Google Scholar 

  18. Suzuki Y, Morioka T, Matsumoto M (1980)Biochim Biophys Acta 619:632–39.

    Google Scholar 

  19. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951)J Biol Chem 193:265–75.

    Google Scholar 

  20. Seyfried TN, Ando S, Yu RK (1978)J Lipid Res 19:538–43.

    Google Scholar 

  21. Gasa S, Makita A, Kinoshita Y (1983)J Biol Chem 258:876–81.

    Google Scholar 

  22. Hirabayashi Y, Nakao T, Matsumoto M, Obata K, Ando S (1988)J Chromatogr 445:377–84.

    Google Scholar 

  23. Wherrett JR (1973)Biochim Biophys Acta 326:63–73.

    Google Scholar 

  24. Nilson O, Mansson JE, Tibblin E, Svennerholm L (1981)FEBS Lett 133:197–200.

    Google Scholar 

  25. Suzuki Y, Suzuki T, Matsunaga M, Matsumoto M (1985)J Biochem 97:1189–99.

    Google Scholar 

  26. Hasegawa A, Hotta K, Kameyama A, Ishida H, Kiso M (1991)J Cabohydr Chem 10:439–59.

    Google Scholar 

  27. Svennerholm L (1957)Biochim Biophys Acta 24:604–11.

    Google Scholar 

  28. Xu G, Suzuki T, Tahara H, Kiso M, Hasegawa A, Suzuki Y (1994)J Biochem 115:202–7.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, University of Shizuoka, School of Pharmaceutical Science, Shizuoka, 422, Shizuoka, Japan

    Guiyun Xu, Takashi Suzuki, Yasuhiro Maejima & Yasuo Suzuki

  2. Shizuoka Prefectural Institute of Animal Health, Fujieda, 426, Shizuoka, Japan

    Tohru Mizoguchi & Mamoru Tsuchiya

  3. Department of Applied Bio-organic Chemistry, Gifu University, Gifu, 501-11, Gifu, Japan

    Makoto Kiso & Akira Hasegawa

Authors
  1. Guiyun Xu
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  2. Takashi Suzuki
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  3. Yasuhiro Maejima
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  4. Tohru Mizoguchi
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  5. Mamoru Tsuchiya
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  6. Makoto Kiso
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  7. Akira Hasegawa
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  8. Yasuo Suzuki
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Xu, G., Suzuki, T., Maejima, Y. et al. Sialidase of swine influenza A viruses: variation of the recognition specificities for sialyl linkages and for the molecular species of sialic acid with the year of isolation. Glycoconjugate J 12, 156–161 (1995). https://doi.org/10.1007/BF00731360

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  • DOI: https://doi.org/10.1007/BF00731360

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