Abstract
We have shown previously that rat liver macrophages (Kupffer cells) express a membrane-bound form of C-reactive protein (mCRP) on their surface which is identical to a galactose-specific particle receptor activity. We now establish the presence of mCRP on human monocyte-macrophages using immunocytochemistry with an anti-neoCRP specific monocloncal antibody and RNA-RNAin situ hybridization to demonstrate the presence of CRP-specific mRNA. Concomitant with mCRP expression, cells exhibit galactose-dependent uptake of particles coated with lactosylated bovine serum albumin. Adhesion experiments on fibronectin-coated surfaces that mCRP on human blood monocytes may act as a selectin-like adhesion molecule, mediating initial carbohydrate-specific contacts which are followed by peptide-specific recognition via integrin receptors.
Similar content being viewed by others
References
Kolb-Bachofen V (1991)Immunobiology 183:133–45.
Volanakis JE, Kaplan MD (1971)Proc Soc Exp Med Biol 136:612–14.
Anderson JK, Stroud RM, Volanakis JE (1978)Fed Proc 37:1495.
Kaplan MH, Volanakis JE (1974)J Immunol 112:2135–47.
Salonen EM, Vartio T, Hedman K, Vaheri A (1984)J Biol Chem 259:1496–1501.
Tseng J, Mortensen RF (1988)Mol Immunol 25:679–86.
Robey FA, Jones KD, Tanaka T, Liu TY (1984)J Biol Chem 259:7311–16.
Du Clos TW, Zlock LT, Rubin RL (1988)J Immunol 141:4266–70.
Higginbotham JD, Heidelberger M, Gotschlich EC (1970)PNAS 67:138–42.
Emsley J, White HE, O'Hara BP, Oliva G, Srinivasan N, Tickle IJ, Blundell TL, Pepys MB, Wood SP (1994)Nature 367:338–45.
Kempka G, Roos RH, Kolb-Bachofen V (1990)J Immunol 144:1004–9.
Egenhofer C, Alsdrorff K, Fehsel K, Kolb-Bachofen V (1993)J Hepatology 18:1216–23.
Potempa LA, Siegel JN, Fiedel BA, Potempa RT, Gewurz H (1987)Mol Immunol 24:531–41.
Zahedi K, Tebo JM, Siripont J, Klimo F, Mortensen RF (1989)J. Immunol 142:2384–92.
Ballou SP, Buniel J, Macintyre SS (1989)J Immunol 142:2708–15.
Kolb-Bachofen V, Abel F (1991)Carbohydr Res 214:201–13.
Villinger B, Kelley DG, Engleman W, Kuhn C, McDonald JA (1981)J Cell Biol 90:711–20.
Hosein B, Bianca C (1985)J Exp Med 162:157–70.
Argraves WS, Suzuki S, Thompson K, Pierschbacher MD, Rouslahti E (1987)J Cell Biol 105:1183–90.
Holers VM, Ruff TG, Parks DL, McDonald JA, Balliard LL, Brown EJ (1989)J Exp Med 169: 1589–605.
Gresham HD, Goodwin JL, Allen PM, Anderson DC, Brown EJ (1989)J Cell Biol 108:1935–43.
Kolb-Bachofen V (1992)J Clin Invest 90:1819–24.
Boyum A (1968)J Clin Lab Invest Suppl 21:77–89.
Woo P, Korenberg JR, Whitehead AS (1985)J Biol Chem 260:13384–88.
Cleveland DW, Lopata MA, McDonald RJ, Cowan NK, Rutter WJ, Kirschner MW (1980)Cell 20:95–105.
Kolb-Bachofen V (1989)In Methods in Enzymology (Ginsburg V), Vol 179. pp. 111–21. San Diego: Academic Press Limited.
Engvall E, Ruoslahti E (1977)Int J Cancer 20:1–5.
Köttgen E, Hell B, Kage A, Tauber R (1992)J Immunol 149:445–53.
Rouslahti E (1988)Ann Rev Biochem 57:375–413.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kolb-Bachofen, V., Puchta-Teudt, N. & Egenhofer, C. Expression of membrane-associated C-reactive protein by human monocytes: indications for a selectin-like activity participating in adhesion. Glycoconjugate J 12, 122–127 (1995). https://doi.org/10.1007/BF00731355
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00731355