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Structural analysis of a glycosylphosphatidylinositol glycolipid ofLeishmania donovani

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Abstract

A glycosylphosphatidylinositol (GPI) glycolipid antigen recognized by sera from patients with visceral leishmaniasis was isolated fromLeishmania donovani promastigotes. The carbohydrate moiety was cleaved from the lipid part by digestion with specific phosphatidylinositol phospholipase C. After separation, structural analysis was carried out on the phosphorylated inositol oligosaccharide and the alkylacyl glycerol. The following major structures were found:

$$\begin{gathered} Man\alpha 1 - 2Man\alpha 1 \hfill \\ \begin{array}{*{20}c} \backslash \\ 6 \\ { Man\alpha } \\ 3 \\ {Man\alpha 1/} \\ \end{array} 1 - 4GlcN\alpha 1 - inositol - O - \begin{array}{*{20}c} O \\ \parallel \\ P \\ | \\ {O^ - } \\ \end{array} - O - _{\begin{array}{*{20}c} {CH_2 } \\ | \\ {CH} \\ | \\ {CH_2 } \\ \end{array} } \begin{array}{*{20}c} {{\text{ - O - CO - (CH}}_{\text{2}} {\text{)}}_n {\text{ - CH}}_{\text{3}} } \\ {{\text{ - O - (CH}}_{\text{2}} {\text{)}}_m {\text{ - CH}}_{\text{3}} } \\ \end{array} \hfill \\ n = 16,18,20,22,24,26 m = 16,17,18,21 \hfill \\ \end{gathered} $$

The presence of the conserved sequence Manα1-2Manα1-6Manα-4GlcN-PI of glycosyl phosphatidylinositol protein anchors in this antigen may be consistent with a precursor role ofLeishmania glycosyl phosphatidylinositol anchored proteins for this glycolipid.

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Author notes

  1. Daniel Sevlever

    Present address: Department of Pharmacology, School of Medicine, Case Western Reserve University, 44106, Cleveland, OH, USA

Authors and Affiliations

  1. Department of Parasitology, The Kuvin Centre for the Study of Infectious and Tropical Diseases, Hebrew University—Hadassah Medical School, P.O. Box 1172, 91010, Jerusalem, Israel

    Daniel Sevlever, Graciela Rosen & Mauricio V. Londner

  2. Department of Carbohydrate Chemistry, University of Lund, Solvegatan 41, S-22370, Lund, Sweden

    Peter Påhlsson

  3. Biocarb Technology AB, S-22370, Lund, Sweden

    Bo Nilsson

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  1. Daniel Sevlever
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  5. Mauricio V. Londner
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Sevlever, D., Påhlsson, P., Rosen, G. et al. Structural analysis of a glycosylphosphatidylinositol glycolipid ofLeishmania donovani . Glycoconjugate J 8, 321–329 (1991). https://doi.org/10.1007/BF00731344

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