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Expression of the T antigen on a T-lymphoid cell line, SupT1

  • Glycoconjagate Journal
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Abstract

We have measured glycosyltransferase activities of SupT1 cells, a T-lymphoid cell line shown to react with autoantibodies in the sera of many HIV patients. Since considerable α-N-acetylgalactosaminyl-transferase and β1, 3 galactosyl-transferase activities were found in SupT1 cells, at least the O-glycan core 1 structure can probably be synthesized. FACS analysis using an anti-T monoclonal antibody showed expression of the T antigen (Gal β1-3 GalNAc). Glycoproteins with the T antigen were isolated by immunoprecipitation with the anti-T antibody from a SupT1 cell lysate labelled metabolically with3H-glucosamine and then analysed by SDS-PAGE. It was revealed that the precipitate contained a glycoprotein with a molecular weight corresponding to that of leukosialin. O-glycans were prepared from the immunoprecipitate by alkaline-borohydride treatment and then fractionated on Bio-Gel P-2, GalNAcOH and Gal-GalNAcOH being identifiedinter alia. These results suggest that an anti-T antibody may be included in the autoantibodies found in HIV-1 infected individuals.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Engineering, Kyoto Sangyo University, Motoyama, Kamigamo, Kita-ku, 603, Kyoto, Japan

    Hiroshi Nakada, Mizue Inoue, Nobuhiro Tanaka & Ikuo Yamashina

  2. Department of Pathology, Research Institute for Microbial Diseases, Osaka University, 565, Suita, Osaka, Japan

    Nobutaka Wakamiya

Authors
  1. Hiroshi Nakada
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  2. Mizue Inoue
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  3. Nobuhiro Tanaka
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  4. Nobutaka Wakamiya
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  5. Ikuo Yamashina
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Nakada, H., Inoue, M., Tanaka, N. et al. Expression of the T antigen on a T-lymphoid cell line, SupT1. Glycoconjugate J 12, 356–359 (1995). https://doi.org/10.1007/BF00731338

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  • DOI: https://doi.org/10.1007/BF00731338

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