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Glycoconjugate Journal

, Volume 12, Issue 3, pp 350–355 | Cite as

Accessible hyaluronan receptors identical to ICAM-1 in mouse mast-cell tumours

  • Stefan Gustafson
  • Tomas Björkman
  • Nina Forsberg
  • Thomas Lind
  • Tarja Wikström
  • Kerstin Lidholt
Glycoconjagate Journal

Abstract

Immunohistochemical studies of the hyaluronan (HA)-receptor (R), originally found on liver endothelial cells (LEC) and related to the intercellular adhesion molecule 1 (ICAM-1), showed that polyclonal antibodies against HARLEC (HA receptor on LEC) also stain structures in mouse mastocytomas, mainly vessels. To test if intravenously administered HA might target the tumour receptorsin vivo, mice carrying an inoculated mastocytoma in one hind leg muscle were injected in the tail vein with125I-tyrosine (T)-labelled HA and killed 75 min after injection when organs and tissues were checked for radioactivity. When doses exceeding the binding capacity of the liver were injected, a significant increase in radioactivity (up to five-fold) within the tumour tissue was found. The weight adjusted difference between control and tumour tissue was greater for smaller tumours, probably due to necrosis in the larger. HA-staining of tumours from animals receiving125I-T-HA, showed HA in areas that also stained weakly for ICAM-1 using monoclonal antibodies. ICAM-1 staining was dramatically increased after hyaluronidase treatment of the sections, indicating that the HA is bound to these receptors and thereby blocks antibody recognition.

Keywords

hyaluronan hyaluronic acid radioactive receptors endothelial cells ICAM-1 mouse tumours targeting in vivo 

Abbreviations

ICAM-1

intercellular adhesion molecule 1

HA

hyaluronan

HARLEC

hyaluronan receptor on liver endothelial cells

MW

molecular weight

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Copyright information

© Chapman & Hall 1995

Authors and Affiliations

  • Stefan Gustafson
    • 1
  • Tomas Björkman
    • 1
  • Nina Forsberg
    • 1
  • Thomas Lind
    • 1
  • Tarja Wikström
    • 1
  • Kerstin Lidholt
    • 1
  1. 1.Department of Medical and Physiological ChemistryUniversity of UppsalaUppsalaSweden

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