Glycoconjugate Journal

, Volume 12, Issue 3, pp 234–240 | Cite as

Changes of β-1,4-N-acetylglucosaminyltransferase III (GnT-III) in patients with leukaemia

  • Masafumi Yoshimura
  • Yoshito Ihara
  • Naoyuki Taniguchi
Glycosylation & Disease Section Special Issue — Blood Glycoconjugates As Markers Of Disease

Abstract

Changes in the activity and transcription of UDP-N-acetyglucosamine: β-d-mannoside β-1,4-N-acetylglucosaminyl-transferase III (GnT-III: EC 2.4.1.144) were investigated in haematological malignancies. GnT-III activity was elevated in patients with chronic myelogeneous leukaemia in blast crisis (CML-BC) and patients with multiple myeloma (MM); whereas most of the normal healthy subjects and patients with other haematological malignancies, including CML in its chronic phase, showed negligible activity. The GnT-III transcript of leukaemic cells from various haematological diseases showed a single band with a similar size. The ratio of GnT-III activity per normalized transcript in CML-BC was considerably higher than in the other conditions, which provided the possibility that in CML-BC the transcript or the enzyme protein might be more stable, or that a post-translational modification of the enzyme might enhance its activity. Furthermore, a lectin blot analysis of patient specimens and a lectin fluorescence study of CML cell lines revealed that E4-PHA binding to surface glycoproteins correlated with GnT-III activity, indicating that more bisecting GlcNAc was added to these glycoproteins, catalysed by elevated GnT-III in CML-BC.

Keywords

N-acetylglucosaminyltransferase III chronic myelogeneous leukaemia E4-PHA 

Abbreviations

GnT-III

UDP-N-acetyglucosamine: β-d-mannoside β-1,4-N-acetylglucosaminyl transferase III

CML

chronic myelogeneous leukaemia

AML

acute myelogeneous leukaemia

ALL

acute lymphoblastic leukaemia

CLL

chronic lymphocytic leukaemia

MM

multiple myeloma

DSA

Datura stramonium

E4-PHA

E4-phytohaemaggultinin

Con A

Concanavalin A; high-performance liquid chromatography

GlcNAc

N-acetylglucosamine

PBS

phosphate-buffered saline

BSA

borine serum albumin

SDS

sodium dodecyl sulfate

SDS-PAGE

SDS-polyacrylamide gel electrophoresis

FITC

fluorescein isothiocyanate

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Copyright information

© Chapman & Hall 1995

Authors and Affiliations

  • Masafumi Yoshimura
    • 1
  • Yoshito Ihara
    • 1
  • Naoyuki Taniguchi
    • 1
  1. 1.Department of BiochemistryOsaka University Medical SchoolSuita, OsakaJapan

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