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Effects of natural complex carbohydrate (citrus pectin) on murine melanoma cell properties related to galectin-3 functions

Glycoconjugate Journal volume 11, pages 527–532 (1994)Cite this article

Abstract

Citrus pectin (CP) and pH-modified citrus pectin (MCP) are highly branched and non-branched complex polysaccharides, respectively, rich in galactoside residues, capable of combining with the carbohydrate-binding domain of galectin-3. We reported previously that intravenous injection of B16-F1 murine melanoma cells with CP or MCP into syngeneic mice resulted in a significant increase or decrease of lung colonization, respectively (Platt D, Raz A (1992)J Natl Cancer Inst 84:438–42). Here we studied the effects of these polysaccharides on cell-cell and cell-matrix interactions mediated by carbohydrate-recognition. MCP, but not CP, inhibited B16-F1 melanoma cells adhesion to laminin and asialofetuin-induced homotypic aggregation. Both polysaccharides inhibited anchorage-independent growth of B16-F1 cells in semisolid medium, i.e. agarose. These results indicate that carbohydrate-recognition by cell surface galectin-3 may be involved in cell-extracellular matrix interaction and play a role in anchorage-independent growth as well as thein vivo embolization of tumour cells.

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Abbreviations

CP:

natural citrus pectin

MCP:

pH-modified CP

EHS:

Englebreth-Holm Swarm

CMF-PBS:

Ca2+-and Mg2+-free phosphate-buffered saline, pH 7.2

HRP:

horseradish peroxidase

ABTS:

2,2′-azino-di(3-ethylbenzthiazoline sulfonic acid

DMEM:

Dulbecco's modified Eagle's minimal essential medium

BSA:

bovine serum albumin

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Affiliations

  1. Cancer Metastasis Program, Michigan Cancer Foundation, 48201, Detroit, MI, USA

    H. Inohara & A. Raz

Authors
  1. H. Inohara
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  2. A. Raz
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Inohara, H., Raz, A. Effects of natural complex carbohydrate (citrus pectin) on murine melanoma cell properties related to galectin-3 functions. Glycoconjugate J 11, 527–532 (1994). https://doi.org/10.1007/BF00731303

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  • DOI: https://doi.org/10.1007/BF00731303

Keywords

  • galectin-3
  • citrus pectin
  • melanoma