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Expression of galectins on microvessel endothelial cells and their involvement in tumour cell adhesion

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Abstract

Lactoside-binding lectins (galectins) with molecular weights of about 14.5 kDa (galectin-1) and 29–35 kDa (galectin-3) bind preferentially to polylactosaminoglycan-containing glycoconjugates and have been found on the surface of tumour cells and implicated in cell-cell and cell-extracellular matrix adhesion and metastasis. We have demonstrated by immunoblotting that both galectin-1 and galectin-3 are present in extracts of endothelial cells cultured from bovine aorta, rat lung, mouse lung and mouse brain microvessels, whereas mouse hepatic sinusoidal endothelial cells expressed primarily galectin-1. These galectins were also localized by indirect immunofluorescent labelling on the surface of the different endothelial cells in culture and by immunohistochemical staining in human tissuesin vivo. Anti-galectin-1 antibodies inhibited the adhesion of liver-preferring murine RAW117-H10 large-cell lymphoma cells to hepatic sinusoidal endothelial cells or lung microvessel endothelial cellsin vitro. The data indicate that galectin-1 is expressed on the extracellular surface of endothelial cells and can mediate in part the adhesion of RAW117-H10 cells to liver microvessel endothelial cells.

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Author information

Author notes

  1. Paula N. Belloni

    Present address: Syntex, Palo Alto, CA, USA

  2. Robert J. Tressler

    Present address: Glycomed, 94501, Alameda, CA, USA

Authors and Affiliations

  1. Department of Tumor Biology, The University of Texas, M. D. Anderson Cancer Center, Houston, 1515 Holcombe Blvd, Box 108, 77030, Texas, USA

    Reuben Lotan, Paula N. Belloni, Robert J. Tressler, Dafna Lotan, Xiao-Chun Xu & Garth L. Nicolson

Authors
  1. Reuben Lotan
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  2. Paula N. Belloni
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  3. Robert J. Tressler
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  4. Dafna Lotan
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  5. Xiao-Chun Xu
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  6. Garth L. Nicolson
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Lotan, R., Belloni, P.N., Tressler, R.J. et al. Expression of galectins on microvessel endothelial cells and their involvement in tumour cell adhesion. Glycoconjugate J 11, 462–468 (1994). https://doi.org/10.1007/BF00731282

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  • DOI: https://doi.org/10.1007/BF00731282

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