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The expression of CMP-NeuAc: Galβ1,4GlcNAc α2,6 sialyltransferase [EC 2.4.99.1] and glycoproteins bearing α2,6-linked sialic acids in human brain tumours

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Abstract

The expression of CMP-NeuAc: Galβ1,4GlcNAc α2,6 sialyltransferase (α2,6-ST) [EC 2.4.99.1] and glycoproteins bearing α2,6-linked sialic acids were examined in primary human brain tumours and cell lines. 79% (19/24) of the meningiomas expressed α2,6-ST mRNA, 42% (10/24) of which showed very high expression. α2,6-ST mRNA expression was undetectable in normal brain tissue. In contrast, only 1/13 of the gliomas examined expressed detectable α2,6-ST mRNA. Metastases to the brain did not express measurable amounts of α2,6-ST mRNA. Less expression was found in malignant (i.e., anaplastic) compared to benign (i.e. meningothelial) meningiomas. Two-dimensional SDS-PAGE of glioma and meningioma proteins, followed bySambucus nigra lectin staining, revealed the presence of a glycoprotein bearing α2,6-linked sialic acids,M r=53 kDa and a pI=7.0 (MEN-1) that appeared in all seven of the meningiomas examined, but was expressed at barely detectable levels, if at all, in seven out of the seven glioblastomas examined. Thus, decreased α2,6-ST expression may play a role in the aggressive nature of anaplastic meningiomas, but appears to be virtually absent in all tumours of glial origin.

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Authors and Affiliations

  1. The Chicago Institute of Neurosurgery and Neuroresearch, 2515 N. Clark St., 60614, Chicago, IL, USA

    Hirotaka Yamamoto, Yoichi Kaneko, David Vandermulen, Donna Kersey, Edward Mkrdichian, Leonard Cerullo, Jan Leestma & Joseph R. Moskal

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  1. Hirotaka Yamamoto
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  2. Yoichi Kaneko
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  3. David Vandermulen
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  4. Donna Kersey
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Yamamoto, H., Kaneko, Y., Vandermulen, D. et al. The expression of CMP-NeuAc: Galβ1,4GlcNAc α2,6 sialyltransferase [EC 2.4.99.1] and glycoproteins bearing α2,6-linked sialic acids in human brain tumours. Glycoconjugate J 12, 848–856 (1995). https://doi.org/10.1007/BF00731247

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  • DOI: https://doi.org/10.1007/BF00731247

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