Glycoconjugate Journal

, Volume 12, Issue 6, pp 795–801 | Cite as

A transgenic mouse line with α-1,3/4-fucosyltransferase cDNA: production and characteristics

  • Hiroya Obama
  • Tadashi Kaname
  • Akiharu Sudou
  • Toyoko Yanagida
  • Shinya Ikematsu
  • Masayuki Ozawa
  • Hiroki Yoshida
  • Reiji Kannagi
  • Ken-Ichi Yamamura
  • Takashi Muramatsu
Article

Abstract

cDNA of human α-1,3/4-fucosyltransferase (Fuc-TIII) was placed under the control of the chicken β-actin promoter and cytomegalovirus enhancer, then introduced into male pronuclei of fertilized mouse eggs. A transgenic mouse line thus obtained exhibited enhanced expression of Lex (4C9) antigen in endothelial cells located in the glomerulus, sinusoidal capillaries of the liver and capillaries of the heart. Furthermore, in the transgenic mice, sialyl dimeric Lex (FH6) and sialyl Lea (2D3), antigens were strongly expressed in the glomerular endothelial cells.

Keywords

transgenic mice α-1,3/4-fucosyltransferase Lex antigen 

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Copyright information

© Chapman & Hall 1995

Authors and Affiliations

  • Hiroya Obama
    • 1
  • Tadashi Kaname
    • 1
    • 4
  • Akiharu Sudou
    • 1
    • 5
  • Toyoko Yanagida
    • 1
    • 2
  • Shinya Ikematsu
    • 1
  • Masayuki Ozawa
    • 1
  • Hiroki Yoshida
    • 3
  • Reiji Kannagi
    • 6
  • Ken-Ichi Yamamura
    • 4
  • Takashi Muramatsu
    • 1
    • 5
  1. 1.Department of BiochemistryKagoshima UniversityKagoshimaJapan
  2. 2.Department OphtalmologyKagoshima UniversityKagoshimaJapan
  3. 3.Department Pathology, Faculty of MedicineKagoshima UniversityKagoshimaJapan
  4. 4.Department of Developmental Genetics, Institute of Molecular Embryology and GeneticsKumamoto University School of MedicineKumamotoJapan
  5. 5.Department of BiochemistryNagoya University School of MedicineNagoyaJapan
  6. 6.Department of PathologyAichi Cancer Center Research InstituteNagoyaJapan

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