Glycoconjugate Journal

, Volume 9, Issue 1, pp 16–20 | Cite as

Enzyme-linked immunosorbent assays for the measurement of blood group A and B glycosyltransferase activities

  • Lakhu M. Keshvara
  • Elizabeth M. Newton
  • A. Heather Good
  • Ole Hindsgaul
  • Monica M. Palcic
Papers

Abstract

ELISA assays have been developed for α(1–3)N-acetylgalactosaminyltransferase (blood group A transferase) and α(1–3)galactosyltransferase (blood group B transferase) activities. In these assays, microtitre plates coated with the bovine serum albumin conjugate of a synthetic Fucα1–2Galβ-R acceptor substrate are incubated with the appropriate nucleotide donor (UDP-GalNAc or UDP-Gal) and human serum as the enzyme source. The resulting trisaccharide products Fucα1–2(GalNAcα1–3)Galβ-R-BSA or Fucα1–2(Galα1–3)Galβ-R-BSA are detected and quantified with monoclonal antibodies selected not to cross-react with the substrate structure. With less than a microliter of human serum, product formation is proportional to enzyme concentration and to time of incubation of up to 90 min.

Keywords

ELISA blood group AN-acetylgalactosaminyltransferase blood group B galactosyltransferase glycosyltransferase assays 

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Copyright information

© Chapman and Hall 1992

Authors and Affiliations

  • Lakhu M. Keshvara
    • 1
    • 2
  • Elizabeth M. Newton
    • 1
    • 2
  • A. Heather Good
    • 3
  • Ole Hindsgaul
    • 2
  • Monica M. Palcic
    • 1
  1. 1.Department of Food ScienceUniversity of AlbertaEdmontonCanada
  2. 2.Department of ChemistryUniversity of AlbertaEdmontonCanada
  3. 3.Chembiomed Ltd., Edmonton Research and Development ParkEdmontonCanada

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