Glycoconjugate Journal

, Volume 11, Issue 2, pp 163–167 | Cite as

The elimination ofO-linked glycans from glycoproteins under non-reducing conditions

  • Catherine A. Cooper
  • Nicolle H. Packer
  • John W. Redmond
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Abstract

A simple procedure is described for the elimination ofO-linked glycans from bovine submaxillary mucin under non-reducing conditions, using triethylamine in aqueous hydrazine. The glycans were isolated as the hydrazones, which were converted to the reducing glycans by exchange with acetone in neutral aqueous solution. The glycan alditols obtained after reduction corresponded to those obtained by the reductive β-elimination ofO-glycans.

Keywords

O-linked sugars mucin glycoprotein β-elimination sugar hydrazones reducing sugars 

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Copyright information

© Chapman & Hall 1994

Authors and Affiliations

  • Catherine A. Cooper
    • 1
  • Nicolle H. Packer
    • 1
  • John W. Redmond
    • 1
  1. 1.School of ChemistryMacquarie UniversityNorth RydeAustralia

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