Glycoconjugate Journal

, Volume 11, Issue 2, pp 105–110 | Cite as

Enzymic synthesis of the trisaccharide core region of the carbohydrate chain ofN-glycoprotein

  • Taichi Usui
  • Masahiro Suzuki
  • Toshinari Sato
  • Hirokazu Kawagishi
  • Kyoko Adachi
  • Hiroshi Sano
Glycopinion Mini-Review

Abstract

Transmannosylation from mannotriose (Manβ1-4Manβ1-4Man) to the 4-position at the nonreducing end N-acetylglucosaminyl residue ofN,N′-diacetylchitobiose was regioselectively induced through the use of β-d-mannanase fromAspergillus niger. The enzyme formed the trisaccharide Manβ1-4GlcNAcβ1-4GlcNAc (3.7% of the enzyme-catalysed net decrease ofN,N′-diacetylchitobiose) from mannotriose as a donor andN,N′-diacetylchitobiose as an acceptor. Mannobiose (Manβ1-4Man) was also shown to be useful as a donor substrate for the desired trisaccharide synthesis.

Keywords

Manβ1-4GlcNAcβ1-4GlcNAc β-d-mannanase in carbohydrate synthesis trisaccharide core ofN-glycoprotein 

Abbreviations

Man

d-mannose

(Mn) (n=1−5)

β-linkedn-mer of mannose

GlcNAc2

2-acetamido-2-deoxy-β-d-glucopyranosyl-(1–4)-2-acetamido-2-deoxy-d-glucose

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Copyright information

© Chapman & Hall 1994

Authors and Affiliations

  • Taichi Usui
    • 1
  • Masahiro Suzuki
    • 1
  • Toshinari Sato
    • 1
  • Hirokazu Kawagishi
    • 1
  • Kyoko Adachi
    • 2
  • Hiroshi Sano
    • 2
  1. 1.Faculty of Agriculture, Department of Applied Biological ChemistryShizuoka UniversityShizuokaJapan
  2. 2.Marine Biotechnology Institute Co., LtdShimizuJapan

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