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Glycoconjugate Journal

, Volume 9, Issue 6, pp 331–335 | Cite as

Serum immunoassay of a small cell lung cancer associated ganglioside: development of a sensitive scintillation proximity assay

  • Tina Pallesen
  • Annette Vangsted
  • Lars Drivsholm
  • Henrik Clausen
  • Jesper Zeuthen
  • Håkan Wallin
Papers

Abstract

We here report an enzyme linked immunosorbent assay (ELISA) and a scintillation proximity assay (SPA) for detection of the ganglioside FucGM1 in sera from small cell lung cancer (SCLC) patients. The SPA was more sensitive and reproducible than the ELISA. In this assay, monoclonal antibodies specific for FucGM1 were bound to SPA particles and incubated with labelled FucGM1 and 100 µl test-serum overnight, and counted in a β-counter. The sensitivity was 0.2 ng. Seven out of twenty sera from SCLC patients were positive, whereas none of twenty sera from healthy individuals were positive for FucGM1. The SPA was more sensitive than the previously reported HPTLC as well as a direct ELISA.

Keywords

SCLC FucGM1 sera immunoassay ELISA scintillation proximity assay 

Abbreviations

MAb

monoclonal antibody

SPA

scintillation proximity assay

HPTLC

high performance thin layer chromatography

SCLC

small cell lung cancer

FucGM1

Fucα1-2Galβ1-3GalNAcβ1-4(NeuAcα2-3)-Galβ1-4Glcβ1-1Cer

ELISA

enzyme linked immunosorbent assay

FCS

foetal calf serum

PBS

phosphate buffered saline

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Copyright information

© Chapman & Hall 1992

Authors and Affiliations

  • Tina Pallesen
    • 2
    • 3
    • 4
    • 1
  • Annette Vangsted
    • 2
    • 3
    • 4
    • 1
  • Lars Drivsholm
    • 2
  • Henrik Clausen
    • 3
  • Jesper Zeuthen
    • 2
    • 3
    • 4
    • 1
  • Håkan Wallin
    • 4
  1. 1.Department of Tumor Cell BiologyThe Fibiger InstituteCopenhagenDenmark
  2. 2.Department of OncologyState University HospitalCopenhagenDenmark
  3. 3.School of DentistryUniversity of CopenhagenCopenhagenDenmark
  4. 4.Department of Environmental CarcinogenesisDanish Cancer SocietyCopenhagenDenmark

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