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Glycoconjugate Journal

, Volume 8, Issue 2, pp 108–115 | Cite as

Oligosaccharide mapping of proteoglycan-bound and xyloside-initiated dermatan sulfate from fibroblasts

  • Lars-Åke Fransson
  • Artur Schmidtchen
  • Lars Cöster
  • Anders Malmström
Papers

Abstract

The copolymeric structure of dermatan sulfate chains synthesized by skin fibroblasts has been examined. Chains initiated onto exogeneousp-nitrophenyl-β-D-xylopyranoside or attached to protein in a large proteoglycan, PG-L, and two small proteoglycans, PG-S1 and PG-S2, have been compared by using high resolution electrophoresis and gel chromatography of oligosaccharides generated by specific enzymatic or chemical degradations. The results confirm that chains attached to PG-L are glucuronate-rich, whereas novel findings indicate that chains attached to either of the two PG-S variants yield closely similar oligosaccharide maps, have approximately equal glucuronate and iduronate content and contain over 90% 4-sulfated disaccharide repeating units. Dermatan sulfate chains built onto xyloside at concentrations of 50 µm and below have a copolymeric structure similar to that of chains from the two PG-S variants. These findings indicate that the polymer-modifying machinery can generate chains with extended iduronate-containing repeats also when the xylose primer is not linked to core protein.

Keywords

Proteoglycans dermatan sulfate oligosaccharide mapping xylosides 

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Copyright information

© Chapman and Hall Ltd 1991

Authors and Affiliations

  • Lars-Åke Fransson
    • 1
  • Artur Schmidtchen
    • 1
  • Lars Cöster
    • 1
  • Anders Malmström
    • 1
  1. 1.Department of Physiological ChemistryUniversity of LundLundSweden

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