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Glycoconjugate Journal

, Volume 8, Issue 2, pp 90–94 | Cite as

A simple synthesis of octyl 3,6-di-O-(α-d-mannopyranosyl)-β-d-mannopyranoside and its use as an acceptor for the assay ofN-acetylglucosaminyltransferase-I activity

  • Kanwal J. Kaur
  • Ole Hindsgaul
Papers

Abstract

A simple synthesis of octyl 3,6-di-O-(α-d-mannopyranosyl)-β-d-mannopyranoside is described. The key features of the synthetic scheme are the formation of the β-mannosidic linkage by 1-O-alkylation of 2,3,4,6-tetra-O-acetyl-α,β-d-mannopyranose with octyl iodide and glycosylation of unprotected octyl β-d-mannopyranoside using limiting acetobromomannose. The trisaccharide is shown to be an acceptor forN-acetylglucosaminyltransferase-I with aKM of 585 µm.

Keywords

N-acetylglucosaminyltransferase-I glycosyltransferase assay core-trimannoside oligosaccharide synthesis 

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Copyright information

© Chapman and Hall Ltd 1991

Authors and Affiliations

  • Kanwal J. Kaur
    • 1
  • Ole Hindsgaul
    • 1
  1. 1.The Department of ChemistryUniversity of AlbertaEdmontonCanada

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