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Routine large-scale electrophoresis for plant breeding. An example with F1 seeds of Brussels sprouts (Brassica oleracea var.gemmifera)

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Summary

A routine method of large-scale electrophoresis is proposed for use in plant breeding. The method can be applied both with research and teaching and does not require sophisticated apparatus. A skilled laboratory technician can thus handle 768 samples a day. Within a series of electrophoretic investigations the individual electrophoresis can be stopped at any time, while the other investigations in the series continue.

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Reference

  • WillsA. B., S. K.Fyfe & E. M.Wiseman, 1979. Testing F1 hybrids ofBrassica oleracea for sibs by seed isoenzyme analysis. Annals of Appl. Biol. 91: 262–270.

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Authors and Affiliations

  1. Department of Plant Breeding (IvP), Agricultural University, Wageningen, the Netherlands

    L. C. J. M. Suurs

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  1. L. C. J. M. Suurs
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Suurs, L.C.J.M. Routine large-scale electrophoresis for plant breeding. An example with F1 seeds of Brussels sprouts (Brassica oleracea var.gemmifera). Euphytica 36, 147–151 (1987). https://doi.org/10.1007/BF00730657

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  • DOI: https://doi.org/10.1007/BF00730657

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