Summary
Extent of heterosis for seed yield is an indicator for transgressive segregation in cross of self-pollinated crops. However, the extent of heterosis is thought to be related to the genetic diversity among the parents. Isoenzymic variation was used for estimating the genetic diversity. Eight varieties of green gram (Vigna radiata) were characterized for esterase, amylases, phosphatases, catalase and peroxidase isoenzymes. Differences in these systems were used to calculate a diversity index (D.I.). Hybrids produced from an inbred pair having higher D.I.'s outyielded those with low D.I.'s. However, the correlation (r) between D.I. and heterosis for seed yield was not consistent in different seasons but some of the desirable crosses could be singled out on the basis of D.I.
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References
BrewbakerJ. L., M. D.Upadhya, Y.Makinen & Macdonald, 1968. Isoenzyme polymorphism in flowering plants III. Gel electrophoresis methods and applications. Physiol. Plant. 21: 930–940.
DavisB. J., 1964. Disc gel electrophoresis. II. Method and application to human serum protein. Ann. N.Y. Acad. Sci. 121: 404–427.
Heidrich-SobrinhoE. & A. R.Cordeiro, 1975. Codominant isoenzymic alleles as markers of genetic diversity correlated with heterosis in maize (Zea mays). Theor. Appl. Genet. 46: 197–199.
HunterR. B. & L. W.Kannenberg, 1971. Isozyme characterization of corn (Zea mays) inbreds and its relationship to single-cross hybrid performance. Can. J. Genet. Cytol. 13: 649–655.
LowryO. H., N. J.Rosenbrough, A. L.Farr & R. J.Randall, 1951. Protein measurement with folin phenol reagent. J. Biol. Chem. 193: 265–275.
MitraR. D., R.Jagannath & C. R.Bhatia, 1970. Disc electrophoresis of analogous enzymes inHordeum. Phytochem. 9: 1843–1850.
MollR. H., J. H.Lonnquist, J. V.Fortuno & E. C.Johnson, 1965. The relationship of heterosis and genetic divergence in maize. Genetics 52: 139–144.
MollR. H., W. H.Salhuana & H. F.Robinson, 1962. Heterosis and genetic diversity in variety crosses of maize. Crop Sci. 2: 197–198.
OrnsteinL., 1964. Disc electrophoresis. I. Background and theory. Ann. N.Y. Acad. Sci 121: 321–340.
ScandaliosJ. G., 1969. Genetic control of multimolecular forms of enzymes in plant. A review. Biochem. Genet. 3: 37–79.
SinghK. B., 1974. Exploitation of heterosis in pulse crops. Proc. 2nd general Congress, SABRAO, New Delhi, 1973. Indian J. Genet. 34: 752–756.
Singh, M., 1980. Genetic and immunochemical analysis of heterosis in green gram (Vigna radiata L. Wilczek). Ph.D. Thesis, Haryana Agric. Univ. Hisar.
SpoffordJ. B., 1969. Heterosis and the evolution of duplications. Am. Nat. 103: 407–432.
StoddartJ. L., 1971. sequential changes in amylase isoenzymes during grains maturation in barley. Planta 97: 70–82.
TsotsisB., 1972. Objectives of industry breeders to make efficient and significant advances in the future. Proc. Ann. Corn Sorghum Res. Conf. 27: 93–107.
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Mittal, R.K., Singh, M. & Maherchandani, N. Isoenzymic diversity index of vigna parents in relation to heterosis for seed yield in green gram (Vigna radiata (L.)Wilczek). Euphytica 36, 61–68 (1987). https://doi.org/10.1007/BF00730648
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DOI: https://doi.org/10.1007/BF00730648