Advertisement

Molecular and General Genetics MGG

, Volume 186, Issue 3, pp 432–438 | Cite as

Suppression of defective-sporulation phenotypes by theBacillus subtilis mutationrev4

  • Robert A. Sharrock
  • Terrance Leighton
Article

Summary

TheB. subtilis intergenic suppressor mutationrev4 suppresses defective-sporulation phenotypes caused by several mutations in RNA polymerase and in components of the ribosome. These suppressible mutations cause either temperature-sensitive sporulation (Spots) or oligosporogenous phenotypes, arresting sporulation at temporal stages 0 to IV. Double mutants containing Spots lesions in both RNA polymerase and the ribosome are also suppressible. In addition to strains altered in the transcription and translation systems, spontaneous Spots mutants and oligosporogenousspoOA andspoOK mutants respond torev4 suppression. Finally, Spots phenocopies, induced in wild-typeB. subtilis by the addition of the antibiotic cerulenin, ethanol, or phenethyl alcohol to sporulating cultures, are alleviated by therev4 mutation, suggesting thatrev4-suppressible sporulation phenotypes may be associated with defective membrane structure or function.

Keywords

Alcohol Double Mutant Membrane Structure Translation System Phenethyl 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bohin JP, Rigomier D, Schaeffer P (1976) Ethanol sensitivity of sporulation inBacillus subtilis: A new tool for the analysis of the sporulation process. J Bacteriol 127:934–940Google Scholar
  2. Caulfield MP, Berkeley RCW, Pepper EA, Melling J (1979) Export of extracellular levansucrase byBacillus subtilis: inhibition by cerulenin and quinacrine. J Bacteriol 138:345–351Google Scholar
  3. Coote JG (1972a) Sporulation inBacillus subtilis. Characterization of oligosporogenous mutants and comparison of their phenotypes with those of asporogenous mutants. J Gen Microbiol 71:1–15Google Scholar
  4. Coote JG (1972b) Sporulation inBacillus subtilis. Genetic analysis of oligosporogenous mutants. J Gen Microbiol 71:17–27Google Scholar
  5. Coote JG, Mandelstam J (1973) Use of constructed double mutants for determining the temporal order of expression of sporulation genes inBacillus subtilis. J Bacteriol 114:1254–1263Google Scholar
  6. Fishman Y, Rottem S, Criti N (1978) Evidence linking penicillinase formation and secretion to lipid metabolism inBacillus licheniformis. J Bacteriol 134:434–439Google Scholar
  7. Gorini L (1960) Informational suppression. In: Roman HL, Sandler LM, Campbell A (eds) Ann Rev Genet, vol 4. Annual Reviews Inc, Palo Alto CA, pp 107–134Google Scholar
  8. Leighton T (1974) Sproulation specific translational discrimination inBacillus subtilis. J Mol Biol 86:855–863Google Scholar
  9. Leighton T (1977) New types of RNA polymerase mutations causing temperature-sensitive sporulation inBacillus subtilis. J Biol Chem 252:268–272Google Scholar
  10. Nielson JBK, Caulfield MP, Lampen JO (1981) Lipoprotein nature ofBacillus licheniformis membrane penicillinase. Proc Natl Acad Sci USA 78:3511–3515Google Scholar
  11. Omura S (1976) The antibiotic cerulenin, a novel tool for biochemistry as an inhibitor of fatty acid synthesis. Bacteriol Rev 40:681–697Google Scholar
  12. Piggot PJ, Coote JG (1976) Genetic aspects of bacterial endospore formation. Bacteriol Rev 40:908–962Google Scholar
  13. Sharrock RA, Leighton T (1981) Intergenic suppressors of temperature-sensitive sporulation inBacillus subtilis are allele non-specific. Mol Gen Genet 183:532–537Google Scholar
  14. Sharrock RA, Leighton T, Wittmann HG (1981) Macrolide and aminoglycoside antibiotic resistance mutations in theBacillus subtilis ribosome resulting in temperature-sensitive sporulation. Mol Gen Genet 183:538–543Google Scholar
  15. Silva MT, Sousa JCF, Macedo MAE, Polonia J, Pareute AM (1976) Effects of phenethyl alcohol onBacillus andStreptococcus. J Bacteriol 127:1359–1369Google Scholar
  16. Sumida-Yasumoto C, Doi RH (1977)Bacillus subtilis ribonucleic acid polymerase mutants conditionally temperature-sensitive at various stages of sporulation. J Bacteriol 129:433–444Google Scholar
  17. Wayne RR, Leighton T (1981) Physiological suppression of conditional sporulation phenotypes inBacillus subtilis RNA polymerase and ribosomal mutants. Mol Gen Genet 183:550–552Google Scholar
  18. Wayne RR, Price CW, Leighton T (1981) Physiological suppression of the temperature-sensitive sporulation defect in aBacillus subtilis RNA polymerase mutant. Mol Gen Genet 183:544–549Google Scholar
  19. Wille WE, Eisenstadt E, Willecke K (1975) Inhibition ofde novo fatty acid synthesis by the antibiotic cerulenin inBacillus subtilis: Effects on citrate-Mg2+ transport and synthesis of macromolecules. Antimicrob Agents Chemother 8:231–237Google Scholar
  20. Young M (1975) Genetic mapping of sporulation operons inBacillus subtilis using a thermosensitive sporulation mutant. J Bacteriol 122:1109–1116Google Scholar
  21. Young M (1976) Use of temperature-sensitive mutants to study gene expression during sporulation inBacillus subtilis. J Bacteriol 126:928–936Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Robert A. Sharrock
    • 1
  • Terrance Leighton
    • 1
  1. 1.Department of Microbiology and ImmunologyUniversity of CaliforniaBerkeleyUSA

Personalised recommendations