Molecular and General Genetics MGG

, Volume 186, Issue 3, pp 432–438 | Cite as

Suppression of defective-sporulation phenotypes by theBacillus subtilis mutationrev4

  • Robert A. Sharrock
  • Terrance Leighton


TheB. subtilis intergenic suppressor mutationrev4 suppresses defective-sporulation phenotypes caused by several mutations in RNA polymerase and in components of the ribosome. These suppressible mutations cause either temperature-sensitive sporulation (Spots) or oligosporogenous phenotypes, arresting sporulation at temporal stages 0 to IV. Double mutants containing Spots lesions in both RNA polymerase and the ribosome are also suppressible. In addition to strains altered in the transcription and translation systems, spontaneous Spots mutants and oligosporogenousspoOA andspoOK mutants respond torev4 suppression. Finally, Spots phenocopies, induced in wild-typeB. subtilis by the addition of the antibiotic cerulenin, ethanol, or phenethyl alcohol to sporulating cultures, are alleviated by therev4 mutation, suggesting thatrev4-suppressible sporulation phenotypes may be associated with defective membrane structure or function.


Alcohol Double Mutant Membrane Structure Translation System Phenethyl 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Robert A. Sharrock
    • 1
  • Terrance Leighton
    • 1
  1. 1.Department of Microbiology and ImmunologyUniversity of CaliforniaBerkeleyUSA

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