Journal of Fluorescence

, Volume 5, Issue 2, pp 155–158 | Cite as

Time-resolved chlorophyll fluorescence of spruce needles under various light conditions

  • Herbert Schneckenburger
  • Werner Schmidt
Fluorescence Sensing


Different branches of a declined spruce were exposed to full sunlight, reduced sunlight (using a fine wire mesh), and natural shadow. Subnanosecond decay kinetics and time-gated fluorescence spectra of individual needles were measured and compared with their chlorophyll concentration. Sunlight-exposed needles showed lower chlorophyll concentrations and higher intensitiesI3 of a long-lived fluorescent component (decay time about 3 ns) than shadow needles. This seems to be due to a reduced energy transfer from the chlorophyll antenna molecules to the reaction centres of Photosystem II. After light reduction to 15–20% during one summer season the chlorophyll concentration increased, whereasI3 decreased, thus proving some recovery of the photosynthetic apparatus.

Key words

Forest decline chlorophyll fluorescence light stress time-resolved spectroscopy 


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Copyright information

© Plenum Publishing Corporation 1995

Authors and Affiliations

  • Herbert Schneckenburger
    • 1
  • Werner Schmidt
    • 1
  1. 1.Department of OptoelectronicsFachhochschule AalenAalenGermany

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