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Fluorescence lifetime-based biosensing of zinc: Origin of the broad dynamic range

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Abstract

Fluorescence lifetime-based chemical sensors have recently been described for applications in medicine, environmental monitoring, and bioprocess control. These sensors transduce the level of the analyte as a change in the apparent fluorescence lifetime of an indicator phase. We have previously developed a wavelength-ratiometric fluorescence biosensor for zinc based on binding of zinc and dansylamide to apo-carbonic anhydrase which exhibited high sensitivity and selectivity. We demonstrate that the apo-carbonic anhydrase/dansylamide indicator system is very well suited for lifetime-based sensing, with a subnanomolar detection limit and greater than 1000-fold dynamic range. The theoretical basis for the wide dynamic range is discussed.

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Authors and Affiliations

  1. Department of Biological Chemistry, University of Maryland School of Medicine, 108 North Greene Street, 21201, Baltimore, Maryland

    Richard B. Thompson & Marcia W. Patchan

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  1. Richard B. Thompson
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  2. Marcia W. Patchan
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Thompson, R.B., Patchan, M.W. Fluorescence lifetime-based biosensing of zinc: Origin of the broad dynamic range. J Fluoresc 5, 123–130 (1995). https://doi.org/10.1007/BF00727528

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