Pflügers Archiv

, Volume 428, Issue 3–4, pp 422–424 | Cite as

Level of expression inXenopus oocytes affects some characteristics of a plant inward-rectifying voltage-gated K+ channel

  • A. -A. Véry
  • C. Bosseux
  • F. Gaymard
  • H. Sentenac
  • J. -B. Thibaud
Short Communication Molecular and Cellular Physiology

Abstract

The plant K+ channel KAT1 shows some similarity to animal voltage-gated channels of the Shaker superfamily. Contrary to these animal counterparts, this plant channel is inwardly rectifying, being gated upon hyperpolarization. Different levels of expression of KAT1 inXenopus oocytes could be obtained by increasing the amount of injected cRNA. The resulting KAT1 gating and sensitivity to external caesium were significantly changed. Similar findings have been published regarding animal voltage-gated channels. The present data show that plant channels may also undergo modification of their activity upon modification of their level of expression.

Key words

potassium channels Arabidopsis thaliana Xenopus oocytes activation kinetics caesium blockade 

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • A. -A. Véry
    • 1
  • C. Bosseux
    • 1
  • F. Gaymard
    • 1
  • H. Sentenac
    • 1
  • J. -B. Thibaud
    • 1
  1. 1.Laboratoire de Biochimie et Physiologie VégétalesCNRS URA 573, ENSA.M-INRAMontpellier cedex 1France

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