Summary
Immunohistochemical localization of epidermal growth factor receptor (EGFR) in normal salivary glands and tumours (108 cases) was studied using a monoclonal antibody. In the normal salivary glands, EGFR was occasionally detected in ductal segments of intercalated, striated, and excretory ducts, but not in acinar cells. The frequency of positive EGFR staining in salivary gland tumours was not high: pleomorphic adenoma, 33.8%; mucoepidermoid tumour, 25.0%; adenolymphoma, 44.4%; and sialoadenocarcinoma, 66.6%. Pleomorphic adenomas showed positive staining for EGFR on the luminal side of luminal cells and in squamous metaplastic cells of tumour tissue. Some modified myoepithelial cells were also reactive whereas outer spindle tumour cells were unstained. Adenolymphomas regularly exhibited positive EGFR staining in the cell membrane; mucoepidermoid carcinoma displayed positive staining in cell membranes in epidermoid tumour cells and cytoplasmic staining in mucous-secreting tumour cells. Sialocarcinomas revealed cell membrane staining and whole cytoplasmic staining for EGFR. The immunohistochemical localization of EGFR could be classified into two types, one the cell membrane-positive type found in epithelial tumour cells, and the second the cytoplasmic positive type seen in normal ductal cells, the luminal tumour cells of pleomorphic adenomas and mucous-secreting tumour cells.
Similar content being viewed by others
References
Caselitz J, Seifert G (1987) New developments in tumor markers and receptors in head and neck tumors. In: Cimino F, Birkmayer GD, Klavins JV, Pimentel E, Salvatore F (eds) Human Tumor Markers, Biology and Clinical Applications. Walter de Gruyter, Berlin New York, pp 731–753
Barka T (1980) Biologically active polypeptides in submandibular gland. J Histochem Cytochem 28:836–859
Berger MS, Gullick WJ, Greenfield C, Evans S, Addis B, Waterfield MD (1987) Epidermal growth factor receptors in lung tumors. J Pathol 152:297–307
Carpenter G, Cohen S (1979) Epidermal growth factor. Annu Revi Biochem 48:193–216
Cowley G, Smith JA, Gusterson B, Hendler F, Ozanne B (1984) The amount of EGF receptor is elevated on squamous cell carcinomas. In: Cancer cells, Vol 1. The Tansformed Phenotype. Cold Spring Harbor, NY, Cold Spring Harbor Laboratory, pp 5–10
Damjanov I, Mildner B, Knowles BB (1986) Immunohistochemical localization of the epidermal growth factor receptor in normal human tissues. Lab Invest 55:588–592
Downward J, Yarden Y, Mayes E, Scrace G, Totty N, Stockmell P, Ullrich A, Schlessinger J, Waterfield MD (1984) Close similarity of epidermal growth factor receptor and v-erb-B oncogene protein sequences. Nature 307:521–527
Elder JB, Williams G, Lacey E, Gregory H (1978) Cellular localization of human urogastrone/epidermal growth factor. Nature 271:466–467
Fitzpatrick SL, Brightwell J, Wittliff JL, Barrows GH, Schultz GS (1984) Epidermoid growth factor binding by breast tumor biopsies and relationship to estrogen receptor and progestin receptor levels. Cancer Res 44:3448–3456
Gullick WJ, Marsden JJ, Whittle N, Ward B, Bobrow L, Waterfield MD (1986) Expression of epidermoid growth factor receptors on human cervical, ovarian and vulval carcinomas. Cancer Res 46:285–292
Gusterson B, Cowley G, Smith JA, Ozanne B (1984) Cellular localization of human epidermal growth factor receptor. Cell Bio Inter Report 8:649–658
Heitz PhU, Kasper M, Van Noorden S, Polak JM, Gregory H, Pearse AGE (1978) Immunohistochemical localization of urogastrone to human duodenal and submandibular glands. Gut 19:408–413
Hendler F, Ozanne B (1984) Human squamous cell lung cancers express increased epidermal growth factor receptors. J Clin Invest 74:647–651
Horne GM, Angus B, Wright C, Needham G, Nicholson S, Harris A, Innes B, Horne CHW (1988) Relationships between oestrogen receptor, epidermal growth factor receptor, ER-D5, and P24 oestrogen regulated protein in human breast cancer. J Pathol 155:143–150
Hwang DL, Tay YC, Shu S, Lev-Ran A (1986) Expression of epidermoid growth factor receptors in human lung tumors. Cancer 58:2260–2263
Kamata N, Chida K, Rikimaru K, Horikoshi M, Enomoto S, Kuroki T (1986) Growth-inhibitory effects of epidermoid growth factor and overexpression of its receptor on human squamous cell carcinomas in culture. Cancer Res 46:1648–1653
Kasselberg AG, Orth DN, Grey ME, Stahlman MT (1985) Immunohistochemical localization of human epidermal growth factor/urogastrone in several human tissue. J Histochem Cytochem 33:315–322
Merlino GT, Xu YH, Richert N, Clark AJ, Ishii S (1985) Elevated epidermal growth factor receptor gene copy number and expression in a squamous carcinoma cell line. J Clinic Invest 75:1077–1079
Möller P, Mechtersheimer G, Kaufmann M, Moldenhauer G, Momburg F, Mattfeld T, Otto HF (1989) Expression of epidermal growth factor receptor in benign and malignant promary tumours of the breast. Virchows Arch [A] 414:157–164
Mori M, Hamada K, Naito R, Tsukitani K, Asano K (1983) Immunohistochemical localization of epidermal growth factor in rodent submandibular glands. Acta Histochem Cytochem 16:536–548
Mori M, Naito R, Tsukitani K, Okada Y, Karo K (1987) Immunohistochemical distribution of human epidermal growth factor in salivary gland tumours. Virchow Arch [A] 411:499–507
Mori M, Naito R, Tsukitani K, Okada Y, Tsujimura T (1989) Widespread distribution of human epidermoid growth factor (hEGF) in human tissues and organs under normal and tumor conditions. — Immunohistochemical studies with the use of polyclonal and monoclonal antibodies —. Acta Histochem Cytochem 22:15–34
Ozanne B, Richards CS, Hendler F, Burns D, Gusterson B (1986) Over-expression of the EGF receptor is a hallmark of squamous cell carcinomas. J Pathol 149:9–14
Ozanne B, Shum A, Richards CS et al. (1985) Evidence for an increase of EGF receptors in epidermoid maligncies. In: Cancer Cell Vol. III. Growth factors and Transformation. Cold Spring Harbor, NY, Cold Spring Harbor laboratory, pp 41–49
Ozawa S, Ueda M, Ando N, Abe O, Shimizu N (1987) High incidence of EGF receptor hyperproduction in esophageal squamous cell carcinomas. Int J Cancer 39:333–337
Ozawa S, Ueda M, Ando N, Abe O, Shimizu N (1988) Epidermal growth factor receptors in cancer tissues of esophagus, lung, pancreas, colorectum, breast and stromach. Jpn J Cancer Res 79:1201–1207
Reifenberger G, Prior R, Deckert M, Wechsler W (1989) Epidermal growth factor receptor expression and growth fraction in human tumours of the nervous system. Virchows Arch [A] 414:147–155
Sainsbury JRC, Malcolm AJ, Appleton DR, Farndon JR, Harris AR (1985) Presence of epidermal growth factor receptor as an indicator of poor prognosis in patients with breast cancer. J Chin Pathol 38:1225–1228
Sakai K, Mori S, Kawamoto T, Taniguchi S, Kobori O, Morioka Y, Kuroki T, Kano K (1986) Expression of epidermoid growth factor receptor on normal human gastric epithelia and gastric cancers. J National Cancer Ins 77:1047–1052
Schreiber AB, Leibermann TA, Lax I, Yarden Y, Schlessinger J (1983) Biological role of epidermal growth factor receptor clustering. Investigation with monoclonal antireceptor antibodies. J Biol Chem 258:864–853
Seifert G, Caselitz J, Hamper K (1987) Receptors and proliferative markers in salivary gland tumors. J Tumor Marker Oncol 2:291–303
Shiurba RA, Eng LF, Vogel H, Lee YL, Horoupian D, Urich H (1988) Epidermal growth factor receptor in meningiomas is expressed predominantly on endothelial cells. Cancer 62:2139–2144
Tatemoto Y, Tsukitani K, Oosumi H, Mori M, Kobayashi K, Kurobe M, Hayashi K (1988) Immunohistochemical localization of human epidermal growth factor/ γ-urogastrone in submandibular glands and in their obstructive lesions. Acta Histochem Cytochem 21:291–300
Tsukitani K, Tatemoto Y, Noda Y, Mori M, Hayashi T, Mori M (1987) Immunohistochemical detection of human epidermal growth factor in submandibular glands and their tumors using a polyclonal antiserum a monoclonal antibody. Histochemistry 87:293–300
Waterfield MD, Mayes ELV, Stroobant P, Bennett PLP, Young S, Goodfellow PN, Banting GS, Ozanne B (1982) An antibody to the human epidermal growth factor receptor. J Cell Biol 20:149–161
Wrba F, Reiner A, Ritzingger E, Holzner JH (1988) Expression of epidermal growth factor receptor (EGFR) on brest cancinomas in relation to growth fractions, estrogen receptor status and morphological criteria. An immunohistochemical study. Pathol Res Pract 183:25–29
Yarden Y, Harari I, Schlessinger J (1985) Purification of an active EGF receptor kinase with monoclonal antireceptor antibodies. J Biol Chem 260:315–319
Yasui W, Sumiyoshi H, Hara J, Kameda T, Ochiai A, Ito H, Tahara E (1988) Expression of epidermal growth factor receptor in human gastric and colonic cancinomas. Cancer Res 48:137–141
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yamada, K., Iwai, K., Okada, Y. et al. Immunohistochemical expression of epidermal growth factor receptor in salivary gland tumours. Vichows Archiv A Pathol Anat 415, 523–531 (1989). https://doi.org/10.1007/BF00718645
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00718645