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Nucleolar grading of breast cancer

Comparative studies on frequency and localization of nucleoli and histology, stage, hormonal receptor status and lectin histochemistry

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Summary

The localization and number of nucleoli of tumour nuclei were examined after conventional histological typing of breast carcinomas (WHO classification), staging, biochemical receptor status, and lectin binding site histochemistry. With increasing histological atypia and grade of malignancy, the frequency of nucleoli increased significantly and their location shifted from the center to the nuclear periphery. These changes correlated with an increase in indices of mitosis and cell loss. The nucleolar size correlated with the increase in the grade of malignancy. Hormone receptor and lectin binding site positive carcinomas were characterized by a low nucleolar frequency and by small nucleoli in central position, whereas carcinomas with high grades of malignancy and negative hormone receptor and lectin binding site status showed large, often multiple nucleoli in eccentricat position at a high frequency. The studies have demonstrated that nucleolar status is an easily practicable histological/cytological method for use in the assessment of prognosis of carcinomas of the breast.

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References

  • Azzopardi JG (1982) Problems in breast pathology. Vol 11: Major problems in pathology. Saunders Comp London Philadelphia Toronto

    Google Scholar 

  • Barnard NY, Hall PA, Lemoine NR, Kadar N (1987) Proliferative index in breast carcinomata determined in situ by Ki 67 immunostaining and its relationship to clinical and pathological variables. J Pathol 152:287–295

    Google Scholar 

  • Black MM, Asire MA (1969) Palpable axillary lymphnodes in cancer of the breast. Structural and biologic considerations. Cancer 23:251–259

    Google Scholar 

  • Bloom HJG, Richardson WW (1957) Histological grading and prognosis in breast cancer: A study of 1409 cases of which 359 have been followed 15 years. Br J Cancer 11:359–377

    Google Scholar 

  • Bondi A, Capucci A, Ghidoni D, Folicaldi S, Valmori V (1989) Nucleolar organizer region-associated proteins (AgNORs), hormone receptors and proliferating cells fraction in breast cancer. Pathol Res Pract 185:21

    Google Scholar 

  • Crocker J, Nar P (1987) Nucleolar organizer regions in lymphomas. J Pathol 151:111–118

    Google Scholar 

  • Crocker J, Skilbeck N (1987) Nucleolar organizer regions in melanocytic lesions: a quantitative study. J Clin Pathol 40:885–889

    Google Scholar 

  • Dizerens P, Anani P, Gómez F, De Gani P (1985) Corrélations entre les récepteurs stéroidiens et la différenciation histopathologique des carcinomes mammaires chez la femme. J Gynecol Obstet Biol Reprod 14:843–859

    Google Scholar 

  • Egan M J, Crocker J (1988) Nucleolar organizer regions in cutaneous tumors. J Pathol 154:247–253

    Google Scholar 

  • Feichter GE, Müller A, Kaufmann M, Haag D, Born I, Kubli F, Goerttler K (1985) DNA - ploidy, DNA synthesis, histological grading, stage and steroid receptor content of breast cancer. Verh Dtsch Ges Pathol 69:143–144

    Google Scholar 

  • Fisher ER, Sass R, Fisher B (1987) Pathologic findings from the national surgical adjuvant breast project. Correlations with concordant and discordant estrogen and progesterone receptors. Cancer 59:1554–1559

    Google Scholar 

  • Franklin WA, Bibbo M, Doria MJ, Dytch HE, Tota J, Desombre E, Wild GL (1987) Quantitation of estrogen receptor content and Ki-67 staining in breast carcinoma by the Micro TICAS Image analysis system. Analyt Quant Cytol Histol 9:279–286

    Google Scholar 

  • Gerdes J, Lelle RJ, Rickartz H, Heidenreich W, Schwarting R, Kurtsiefer L, Stauch G, Stein H (1986) Growth fractions in breast cancers determined in situ with monoclonal antibody Ki 67. J Clin Pathol 39:977–980

    Google Scholar 

  • Guazzi A, Bozzetti C, Riva MI, Zaffe D, Cocconi G (1985) Relationship between estrogen receptor concentration and cytomorphometry in breast cancer. Cancer 56:1972–1976

    Google Scholar 

  • Hall PA, Crocker J, Watts A, Stausfeld AG (1988) A comparison of nucleolar organizer region staining and Ki-67 immunostaining in non-Hodgkin's lymphoma. Histopathology 12:247–253

    Google Scholar 

  • Helpap B (1988a) Frequency and localization of nucleoli in nuclei from prostatic carcinoma and atypical hyperplasia. Histopathology 13:203–211

    Google Scholar 

  • Helpap B (1988b) Vergleich von Nukleolenstatus sowie Histologie, Grading und Hormonrezeptorstatus beim Mammakarzinom. Verh Dtsch Ges Pathol 72:595

    Google Scholar 

  • Helpap B (1989) Pathologie der ableitenden Harnwege und der Prostata. Springer Verlag, Berlin Heidelberg New York London Paris Tokyo, pp 255–263

    Google Scholar 

  • Hermanek P, Sobin LH (1987) TNM Classification of malignant tumours. Fourth, fully revised edition. Springer-Verlag, Berlin Heidelberg New York London Paris Tokyo

    Google Scholar 

  • Klinga K, Kaufmann W, Runnebaum B, Kubli F (1982) Steroidhormonrezeptoren beim Mammakarzinom. Dtsch Med Wschr 107:313–315

    Google Scholar 

  • Lee AK, Rosen PP, de Lellis RA, Saigo PE, Gangi MD, Groshen S, Bagin R, Wolfe HJ (1985) Tumor marker expression in breast carcinomas and relationship to prognosis. An immunohistochemical study. Am J Clin Pathol 84:687–696

    Google Scholar 

  • Lelle RJ, Heidenreich W, Stauch G, Gerdes J (1987) The Correlation of growth factors with histological grading and lymph node status in human mammary carcinoma. Cancer 59:83–88

    Google Scholar 

  • Lesser ML, Rosen PP, Senie RT, Duthie K, Menendez-Botet C, Schwartz MK (1981) Estrogen and progesterone receptors in breast carcinoma. Correlations with epidemiology and pathology. Cancer 48:299–309

    Google Scholar 

  • Mattfeld T, Neurohr W, Müller A, Klinga K (1985) Morphometric correlates of steroid receptor content in invasive ductal breast cancer. Verh Dtsch Ges Pathol 69:350–355

    Google Scholar 

  • McGurrin JF, Doria MI, Dawson PJ, Karrison Th, Stein HO, Franklin WA (1987) Assessment of tumor cell kinetics by immunohistochemistry in carcinoma of breast. Cancer 59:1744–1750

    Google Scholar 

  • Meyer JS, Hixton B (1979) Advanced stage and early relapse of breast carcinomas associated with high thymidine labeling indices. Cancer Res 39:4042–4047

    Google Scholar 

  • Meyer JS, Friedman E, McCrate M, Bauer W (1983) Prediction of early course of breast carcinoma by thymidine labeling. Cancer 51:1879–1886

    Google Scholar 

  • Meyer JS, Bauer WC, Rao BR (1978) Subpopulations of breast carcinoma defined by S-phase fraction, morphology and estrogen receptor content. Lab Invest 39:225–235

    Google Scholar 

  • Meyer JS, Rao BR, Stevens SC, White WL (1977) Low incidence of estrogen receptor in breast carcinomas with rapid rates of cellular replication. Cancer 40:2290–2298

    Google Scholar 

  • Meyer JS, Lee LY (1980) Relationships of S-phase fraction of breast carcinoma in relapse to duration of remission, estrogen receptor content, therapeutic responsiveness and duration of survival. Cancer Res 40:1890–1896

    Google Scholar 

  • Möller P, Mechtersheimer G, Kaufmann M, Moldenhauer G, Momberg F, Mattfeld T, Otto HF (1989) Expression of epidermal growth factor receptor in benign and malignant primary tumours of the breast. Virchows Arch [A] 414:157–164

    Google Scholar 

  • Moran RE, Black MM, Alpert L, Straus MJ (1984) Correlation of cell-cycle kinetics, hormone receptors, histopathology, and nodal status in human breast cancer. Cancer 54:1586–1590

    Google Scholar 

  • Müller HA, Altenähr E, Böcking A, Dhom G, Faul P, Göttinger H, Helpap B, Hohbach C, Kastendieck H, Leistenschneider W (1980) Über Klassifikation und Grading des Prostatakarzinoms. Verh Dtsch Ges Pathol 64:609–611

    Google Scholar 

  • Müller-Holzner E, Fend F, Dietze O, Müller LCh, Wiegel J, Mikuz G (1985) DNA-Zytophotometrie und PNA-Inkubation aus Ermittlung prognostischer Faktoren beim Mammakarzinom. Verh Dtsch Ges Pathol 69:544

    Google Scholar 

  • Parl FF, Wagner RK (1980) The histopathological evaluation of human breast cancers in correlation with estrogen receptor values. Cancer 46:362–367

    Google Scholar 

  • Parl FF, Schmidt BP, Dupont WD, Wagner RK (1984) Prognostic significance of estrogen receptor status in breast cancer in relation to tumor stage, axillary node metastasis, and histopathologic grading. Cancer 54:2237–2242

    Google Scholar 

  • Rank F, Dombernowsky P, Jespersen NChB, Pedersen EV, Keiding N (1987) Histologic malignancy grading of invasive ductal breast carcinoma. A regression analysis of prognostic factors in low-risk carcinomas from a multicenter trial. Cancer 60:1299–1305

    Google Scholar 

  • Reiner A, Holzner JH, Reiner G, Spona J, Schemper M, Kolb R, Jakesz R (1985) Histologic grading and prognosis in breast cancer. Verh Dtsch Ges Pathol 69:365–369

    Google Scholar 

  • Reiner A, Kolb R, Reiner G, Jakesz R, Schemper M, Spona J (1987) Prognostic significance of steroid hormone receptors and histological characterization of human breast cancer. J Cancer Res Clin Oncol 113:285–290

    Google Scholar 

  • Reiner A, Reiner G, Spona J, Schemper M, Holzner JH (1988) Histopathologic characterization of human breast cancer in correlation with estrogen receptor status. Cancer 61:1149–1154

    Google Scholar 

  • Remvikos Y, Magdelénat H, Zajdela A (1988) DNA flow cytometry applied to fine needle sampling of human breast cancer. Cancer 61:1629–1634

    Google Scholar 

  • Schenck U, Burger G, Jütting U, Eiermann W (1985) Cellular findings and hormone receptor status — visual and image analytical analysis of breast carcinomas. Verh Dtsch Ges Pathol 69:529–531

    Google Scholar 

  • Schenck U, Burger G, Jütting U, Eiermann W (1986) Zytomorphologisches Grading und Hormonrezeptorstatus beim Mammakarzinom. Visuelle Untersuchungen an Ausstrichpräparaten. Dtsch med Wschr 111:1949–1953

    Google Scholar 

  • Silvestrini R, Sanfillipo O, Tedesco G (1974) Kinetics of human mammary carcinomas and their correlation with the cancer and the host characteristics. Cancer 34:1252–1258

    Google Scholar 

  • Smith R, Crocker J (1988) Evaluation of nucleolar region-associated proteins in breast manignancy. Histopathology 12:113–125

    Google Scholar 

  • Stiens R, Klotz D (1985) The relation between proliferation and regression in breast with special emphasis on cancer. Verh Dtsch Ges Pathol 69:274–278

    Google Scholar 

  • Straus MJ, Moran RE, Muller RE, Wotiz HH (1982) Estrogen receptor heterogenity and the relationship between estrogen receptor and the tritiated thymidine labeling index in human breast cancer. Oncology 39:197–200

    Google Scholar 

  • Tubiana M, Pejovic MH, Chavaudra N, Contesso G, Malaise EP (1984) The long-term prognostic significance of the thymidine labelling index in breast cancer. Int J Cancer 33:441–445

    Google Scholar 

  • Walker RA (1988) The histopathological evaluation of nucleolar organizer region proteins. Histopathology 12:221–223

    Google Scholar 

  • Weintraub J, Weintraub D, Redard M, Vassilakos P (1987) Evaluation of estrogen receptors by immunocytochemistry on fine-needle aspiration biopsy specimens from breast tumors. Cancer 60:1163–1172

    Google Scholar 

  • WHO (1981) Histological typing of breast tumours No 2. Geneva

  • Wrba F, Chott A, Reiner A, Reiner G, Ritzinger E, Holzner JH (1987) Ki 67-Immunoreaktivität in primären Mammakarzinomen. Eine histochemische Studie. Berichte Pathol 105:246–247

    Google Scholar 

  • Wrba F, Reiner A, Ritzinger E, Holzner JH, Reiner G (1988) Expression of epidermal growth factor receptors (EGFR) on breast carcinomas in relation to growth factors, estrogen receptors status and morphological criteria. An immunohistochemical study. Pathol Res Pract 183:25–29

    Google Scholar 

  • Wrba F, Reiner A, Markis-Ritzinger E, Holzner JH, Reiner G, Spona J (1988) Prognostic significance of immunohistochemical parameters in breast carcinomas. Pathol Res Pract 183:277–283

    Google Scholar 

  • Wyllie AH (1987) Apoptosis: Cell death in tissue regulation. J Pathol 153:313–316

    Google Scholar 

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  1. Department of Pathology, Academic Instructional Hospital of the University of Freiburg, PO Box 720, D-7700, Singen, Germany

    Burkhard Helpap

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  1. Burkhard Helpap
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Helpap, B. Nucleolar grading of breast cancer. Vichows Archiv A Pathol Anat 415, 501–508 (1989). https://doi.org/10.1007/BF00718643

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