Plant Cell Reports

, Volume 8, Issue 2, pp 120–123 | Cite as

Cell differentiation in protoplast cultures from embryogenic callus ofAbies alba L.

  • Hermann Lang
  • Hans Willy Kohlenbach
Article

Abstract

Protoplasts were isolated from embryogenic callus ofAbies alba L. which originated from immature seeds. The protoplasts were immobilized in alginate beads in order to follow the development of single protoplasts.

Surrounding culture medium was modified from Kao and Michayluk (1975). After cell wall regeneration subsequent cell divisions lead to the formation of colonies showing an early differentiation of small meristematic cells and large vacuolated “suspensor-type” cells.

Keywords

Cell Wall Cell Division Cell Differentiation Alba Embryogenic Callus 

Abbreviations

6-BA

N6-benzyladenine

IAA

3-indoleacetic acid (potassium salt)

KM

Kao and Michayluk (1975)

MES

2-(N-morpholino)ethanesulfonic acid

NAA

1-naphthalene acetic acid (sodium salt)

PVP

Polyvinylpyrrolidone

SH

Schenk and Hildebrandt (1972)

Tween 80

Polyoxyethylene- sorbitan - monooleate

WPM

(woody plant medium), Lloyd and McCown (1981)

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Hermann Lang
    • 1
  • Hans Willy Kohlenbach
    • 1
  1. 1.Botanisches Institut der Johann Wolfgang Goethe-UniversitätFrankfurt am MainFederal Republic of Germany

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