Skip to main content
SpringerLink
Log in

Transformed calli obtained by direct gene transfer into sunflower protoplasts

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Helianthus annuus protoplasts were transformed with the plasmid pCaMVNEO (Frommet al. 1986) conferring kanamycin resistance to plant. Transformed calli were selected with a frequency of 4 calli for 106 treated protoplasts. DNA was extracted from kanamycin resistant calli. Analysis of this DNA shows the presence of the NPTII gene.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BAP:

6-benzylaminopurine

2,4-D:

2,4 dichlorophenoxyacetic acid

IAA:

Indole-3-acetic acid

NAA:

1-naphtalenoacetic acid

NPT:

Neomycin phosphotransferase

PEG:

Polyethyleneglycol

References

  • Bergounioux C., Perennes C., Brown S., Sarda C. and Gadal P. (1988) Protoplasma 142: 127–136.

    Google Scholar 

  • Bohorova NE., Atanassov A. and Georgieva-Todorova J. (1985) Z. Pflanzenzüchtg. 95: 37–44.

    Google Scholar 

  • Everett N.P., Robinson K.E.P. and Mascarenhas D. (1987) Bio Technology 5: 1201–1204.

    Google Scholar 

  • Finer J.J. (1987) Plant Cell Rep. 6: 372–374.

    Google Scholar 

  • Fraley R.T., Rogers S.G., Horsch R.B. and Barry G.F; (1986) Molecular developmental biology. Ed: Bogorad L. Alan R. Liss Inc. p.15–26.

  • Freyssinet M. and Freyssinet G. (1988) Plant Sci. 56: 177–181.

    Google Scholar 

  • Fromm M.E., Taylor L.P. and Walbot V. (1986) Nature 319: 791–793.

    PubMed  Google Scholar 

  • Goldsbrough P.B., Gelvin S.B. and Larkins B.A. (1986) Mol. Gen. Genet. 202: 374–381.

    Google Scholar 

  • Greco B., Tanzarella O.A., Carrozzo G. and Blanco A. (1984) Plant Sci. Lett. 36: 73–74.

    Google Scholar 

  • Heller R. (1953) Ann. Sc. Nat. Bot. Biol. Veg. 14: 1–223.

    Google Scholar 

  • Hellmer G., Casdaban M., Bevan M., Kayes L. and Chilton M.D. (1984) Bio Technology 2: 520–527

    Google Scholar 

  • Maniatis T., Fritsch E.F. and Sambrook J. (1982) Molecular Cloning. Cold Spring Harbor. NY. 7th edition.

    Google Scholar 

  • Moyne A.L., Thor V., Pellissier B., Bergounioux C., Freyssinet G. and Gadal P. (1988) Plant Cell Rep. 7: 437–440.

    Google Scholar 

  • Murashige T. and Skoog F. (1962) Physiol. Plant 15: 473–497.

    Google Scholar 

  • Negrutiu I., Shillito R., Potrykus I., Biasini G. and Sala F. (1987) Plant Mol. Biol. 8: 363–373.

    Google Scholar 

  • Paterson K.E. and Everett N.P. (1985) Plant Sci. 42: 125–132.

    Google Scholar 

  • Paszkowski J., Shillito R.D., Saul M., Mandak V., Hohn T., Hohn B. and Potrykus I. (1984) EMBO J 3: 2717–2722.

    Google Scholar 

  • Potrykus I., Shillito R.D., Saul M.W. and Paszkowski J. (1985) Plant Mol. Biol. Reporter 3: 117–128.

    Google Scholar 

  • Sadhu M.K. (1974) J. Exp. Biol.12: 110–111

    Google Scholar 

  • Shillito R.D., Paszkowski J. and Potrykus I. (1983) Plant Cell Rep. 2: 244–247.

    Article  Google Scholar 

  • Southern E. (1975) J. Mol. Biol. 98: 503–517.

    PubMed  Google Scholar 

  • Tagu D., Bergounioux C., Cretin C., Perennes C. and Gadal P. (1988) Protoplasma 146: 101–105.

    Google Scholar 

  • Wilcox Mc Cann A., Cooley G. and Van Dreser J. (1988) Plant Cell Tissue Organ Culture 14: 103–110.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire de Physiologie Végétale Moléculaire, Université Paris-Sud, bâtiment 430, F 91405, Orsay Cedex, France

    Anne-Laure Moyne, Denis Tagu, Véronique Thor, Catherine Bergounioux & Pierre Gadal

  2. Rhône-Poulenc Agrochimie, Biologie Moléculaire et Cellulaire Végétale, 14-20 rue Pierre Baizet, BP 9163, F 69263, Lyon Cedex 09, France

    Georges Freyssinet

Authors
  1. Anne-Laure Moyne
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Denis Tagu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Véronique Thor
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Catherine Bergounioux
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Georges Freyssinet
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Pierre Gadal
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by I. K. Vasil

Rights and permissions

Reprints and permissions

About this article

Cite this article

Moyne, AL., Tagu, D., Thor, V. et al. Transformed calli obtained by direct gene transfer into sunflower protoplasts. Plant Cell Reports 8, 97–100 (1989). https://doi.org/10.1007/BF00716848

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00716848

Key Words

Search

Navigation