Plant Cell Reports

, Volume 8, Issue 2, pp 86–89 | Cite as

Electroporation stimulates tranformation of freshly isolated cell suspension protoplasts ofSolanum dulcamara byAgrobacterium

  • P. K. Chand
  • E. L. Rech
  • T. J. Golds
  • J. B. Power
  • M. R. Davey
Article

Summary

Freshly isolated cell suspension protoplasts ofSolanum dulcamara were mixed withAgrobacterium rhizogenes, allowed to settle for 2 h, exposed to electrical pulses and further incubated for 2h. Two pulses of 600 V cm−1 for 2 msec separated by 15 sec produced transformed colonies at relative and absolute transformation frequencies which were 3–4 and 10 fold greater than those obtained by co-cultivation of 3 days old protoplast-derived cells with bacteria. Transformed colonies were not produced when freshly isolated protoplasts were mixed withAgrobacterium but not electroporated. Biochemical analysis confirmed the transgenic nature of plants regenerated from protoplast-derived tissues.

Keywords

Cell Suspension Biochemical Analysis Electrical Pulse Transformation Frequency Transgenic Nature 

Abbreviations

MS

Murashige and Skoog (1962)

NPTII

Neomycin phosphotransferase II

SDS

Sodium dodecylsulphate

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • P. K. Chand
    • 1
  • E. L. Rech
    • 1
  • T. J. Golds
    • 1
  • J. B. Power
    • 1
  • M. R. Davey
    • 1
  1. 1.Plant Genetic Manipulation Group, Department of BotanyUniversity of NottinghamNottinghamUnited Kingdom

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