Skip to main content
Log in

Chromosome elimination and mutation in tetraploid somatic hybrids ofSolanum tuberosum andSolanum phureja

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

The hybridity of eleven somatic hybrids between a diploidS. tuberosum and a diploidS. phureja clone could be verified because the parent karyotypes differed in their C-banding patterns. The hybrids were hypotetraploid and some carried structurally rearranged chromosomes and/or minute centric fragments. The nucleolar chromosomes ofS. phureja were eliminated preferentially. The function of the remaining nucleolar organizer regions was not suppressed. Nuclear DNA content was correlated with the hypotetraploid chromosome numbers.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Austin S, Ehlenfeldt MK, Baer MA, Helgeson JP (1986) Somatic hybrids produced by protoplast fusion betweenS. tuberosum andS. brevidens: phenotypic variation under field conditions. Theor Appl Genet 71: 682–690

    Google Scholar 

  • Barsby TL, Shephard JF, Kemble RJ, Wong R (1984) Somatic hybridization in the genusSolanum: S. tuberosum andS. brevidens. Plant Cell Reports 3: 165–167

    Google Scholar 

  • Butenko RG, Kuchko AA (1980) Somatic hybridization ofSolanum tuberosum L. andSolanum chacoense Bitt. by protoplast fusion. In: Ferenczy L, Farkas GL (eds) Advances in Protoplast Research. Akad Kiacho, Budapest, pp. 293–300

    Google Scholar 

  • Cowell JK (1982) Double minutes and homogeneously staining regions: Gene amplification in mammalian cells. Ann Rev Genet 16: 21–59

    PubMed  Google Scholar 

  • D'Amato F (1985) Cytogenetics of plant cell and tissue cultures and their regenerates. CRC Crit Rev Plant Sci 3: 73–112

    Google Scholar 

  • Ehlenfeldt MK, Helgeson JP (1987) Fertility of somatic hybrids from protoplast fusions ofSolanum brevidens andS. tuberosum. Theor Appl Genet 73: 395–402

    Google Scholar 

  • Fahleson J, Dixelius J, Sundberg E, Glimelius K (1988) Correlation between flow cytometric determination of nuclear DNA content and chromosome number in somatic hybrids withinBrassicaceae. Plant Cell Reports 7: 74–77

    Google Scholar 

  • Fish N, Karp A, Jones MGK (1988) Production of somatic hybrids by electrofusion inSolanum. Theor Appl Genet 76: 260–266

    Google Scholar 

  • Jacobsen E (1987) Genetic diversity in protoplast- and cell-derived plants of potato. In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry. Vol. 3: Potato. Springer, Berlin, Heidelberg, pp. 358–374

    Google Scholar 

  • Kodama Y, Yoshida MC, Sasaki M (1980) An improved silver staining technique for nucleolus organizer regions by using nylon cloth. Jpn J Human Genet 25: 229–233

    Google Scholar 

  • Miller DA, Dev VG, Tantravahi R, Miller OJ (1976) Suppression of human nucleolus organizer activity in mouse-human somatic hybrid cells. Exp Cell Res 101: 235–243

    PubMed  Google Scholar 

  • Pijnacker LP, Ferwerda MA (1984) Giemsa C-banding of potato chromosomes. Can J Genet Cytol 26: 415–419

    Google Scholar 

  • Pijnacker LP, Ferwerda MA, Puite KJ, Roest S (1987) Elimination ofSolanum phureja nucleolar chromosomes inS. tuberosum +S. phureja somatic hybrids. Theor Appl Genet 73: 878–882

    Google Scholar 

  • Puite KJ, Roest S, Pijnacker LP (1986) Somatic hybrid potato plants after electrofusion of diploidSolanum tuberosum andSolanum phureja. Plant Cell Reports 5: 262–265

    Google Scholar 

  • Puite KJ, Ten Broeke W, Schaart JG (1988) Inhibition of cell wall synthesis improves flow cytometric sorting of potato heterofusions resulting in hybrid plants. Plant Sci 56: 61–68

    Google Scholar 

  • Reeder RH (1985) Mechanisms of nucleolar dominance in animals and plants. J Cell Biol 101: 2013–2016

    PubMed  Google Scholar 

  • Sree Ramulu K (1986) Case histories of genetic variability in vitro: potato. In: Vasil IK (ed) Cell culture and somatic cell genetics of plants. Vol 3: Plant regeneration and genetic variability Acad Press, New York, pp. 449–473

    Google Scholar 

  • Sree Ramulu K (1987) Genetic instability during plant regeneration in potato: Origin and implications. Plant Physiol (Life Sci Adv) 6: 211–218

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Communicated by I. Potrykus

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pijnacker, L.P., Ferwerda, M.A., Puite, K.J. et al. Chromosome elimination and mutation in tetraploid somatic hybrids ofSolanum tuberosum andSolanum phureja . Plant Cell Reports 8, 82–85 (1989). https://doi.org/10.1007/BF00716844

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00716844

Keywords

Navigation