Abstract
Hairy root cultures were obtained following inoculation of the stems of sterile plantlets of aDatura candida hybrid withAgrobacterium rhizogenes. The scopolamine and hyoscyamine content was quantified by HPLC and compared with the non-transformed plants. The alkaloid yield (0.68% dry weight) obtained with the hairy roots was 1.6 and 2.6 times the amount found in the aerial parts and in the roots of the parent plants, respectively. Only a small proportion of alkaloids was released into the growth medium. Scopclamine was the principal alkaloid and the scopolamine/hyoscyamine ratio of ca. 5:1 makes these hairy roct cultures worthy of consideration as a source of scopolamine.
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References
El-Dabbas S W, Evans W C (1982) Planta Medica 44: 184–185
El-Imam Y M A (1983) Ph.D. Thesis, University of Nottingham, p. 150
Ellis J G, Ryder M H, Tate M E (1984) Mol.Gen.Genet. 195: 466–473
Flores H, Filner P (1985) Plant Physiol. 77: 4 suppl. 12
Fraley R T, Rogers S G, Horsch R B (1986) In: CRC Critical Reviews in Plant Sciences, Vol 4, Issue 1, pp 1–46
Hamill J D, Parr A J, Rhodes M J C, Robins R J, Walton N J (1987) Bio/Technology 5: 800–804
Jaziri M, Legros M, Homes J, Vanhaelen M (1988) Phytochemistry 27: 419–420
Jung G, Tepfer D (1987) Plant Science 50: 145–151
Kamada H, Okamura N, Satake M, Harada H, Shimomura K (1986) Plant Cell Reports 5: 239–242
Mano Y, Nabeshima S, Matsui C, Ohkawa H (1986) Agric. Biol. Chem. 50: 2715–2722
Nabeshima S, Mano Y, Ohkawa H (1986) Symbiosis 2: 11–18
Ondrej M, Protiva J (1987) Biologia Plantarum (Praha) 29: 241–246
Payne J, Hamill J D, Robins R J, Rhodes M J C (1987) Planta Medica 53: 474–478
Petit A, David C, Dahl G A, Ellis J G, Guyon P, Casse-Delbart F, Tempe J (1983) Mol. Gen. Genet. 190: 204–214
Stahl E (1969) Thin-Layer Chromatography, Springer, Berlin Heidelberg New York
Tepfer D (1984) Cell 37: 959–967
Vervliet G, Holsters M, Teuchy H, Montagu M van, Shell J (1975) J. gen. Virol. 26: 33–48
White F F, Ghidossi G, Gordon M P, Nester E W (1982) Proc. Natl. Acad. Sci. USA 79: 3193–3197
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Christen, P., Roberts, M.F., Phillipson, J.D. et al. High-yield production of tropane alkaloids by hairy-root cultures of aDatura candida hybrid. Plant Cell Reports 8, 75–77 (1989). https://doi.org/10.1007/BF00716842
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DOI: https://doi.org/10.1007/BF00716842