Abstract
Isozyme analyses were carried out on protein extracts of non-embryogenic and embryogenic callus fromZea mays L., using polyacrylamide gel electrophoresis. We examined the isozyme patterns of glutamate dehydrogenase, peroxidase and acid phosphatase for their utility as biochemical markers of maize embryogenic callus cultures. These isozyme systems were also used to examine possible correlations between isozymes and different stages of regeneration. The zymograms of peroxidase and glutamate dehydrogenase differed for non-embryogenic and embryogenic callus. Further, some isozymes were correlated with the morphological appearance of the tissue while others seemed to be involved with the duration of the culture period. Using the same enzyme assays on fresh tissue samples we were able to test the three enzymes as cytochemical markers in embryogenic cultures. Glutamate dehydrogenase proved to be most successful to discriminate embryogenic from non-embryogenic cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ammirato PV (1983) In: Evans DA, Sharp WR, Ammirato PV, Yamada Y (eds) Handbook of plant cell culture, McMillan Publ. Co., New York, pp 82–123
Armstrong CL, Green CE (1985) Planta 164: 207–214
Carlberg I, Jonsson L, Bergenstrahle A, Soderhäll K (1987) Plant Physiol. 84: 197–200
Chawla HS (1988) Plant Cell Tiss. Org. Cult. 12: 299–304
Chen LJ, Luthe DS (1987) Plant Sci. 48: 181–188
Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY, Bi FY (1975) Sci. Sin. 18: 659–668
Everett NP, Wach MJ, Ashworth DJ (1985) Plant Sci. 41: 133–140.
Flick CE, Evans DA, Sharp WR (1983) In: Evans DA, Sharp WR, Ammirato PV, Yamada Y (eds) Handbook of plant cell culture, MacMillan Publish. Co., New York, pp 13–81
Fransz PF (1988) Cytodifferentiation during callus initiation and somatic embryogenesis in Zea mays L. Ph.D. Thesis, Agricultural University, Wageningen.
Fransz PF, Schel JHN (1987) Acta Bot. Neerl. 36: 247–260
Kay LE, Basile DV (1987) Plant Physiol. 84: 99–105
Khavkin EE, Misharin SI, Ivanov VN, Danovich KN (1977) Planta 135: 225–231
Lees EM, Dennis DT (1981) Plant Physiol. 68: 827–830.
Lörz H, Göbel E, Brown P (1988) Plant Breeding 100: 1–25
Maddock SE (1985) In: Bright SWJ, Jones MGK (eds) Cereal tissue and cell culture, Martinus Nijhoff/Dr. W. Junk Publishers, Dordrecht, Boston, pp 175–205
Malmberg R, Messing J, Sussex IM (1985) Molecular Biology of Plants. A laboratory course manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
Murashige T, Skoog F (1962) Physiol.Plant. 15: 473–497
Negrutiu I, Jacobs M, Gaspar T (1979) Z. Pflanzenphysiol. 91: 119–126
Scandalios JG (1974) Ann Rev. Plant Physiol 25: 225–258
Scandalios JG, Sörensen JC (1977) In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue and organ culture, Springer Verlag, Berlin, pp 719–730
Stirn S, Jacobsen HJ (1987) Plant Cell Reports 6: 50–54
Suurs LCJM, Jongedijk E, Tan MMC (1989) Euphytica 39, in the press
Tomes DT (1985) In: Bright SWJ, Jones MGK (eds) Cereal tissue and cell culture, Martinus Nijhoff/Dr. W. Junk Publishers, Dordrecht, Boston, pp 175–205
Vallejos CE (1983) In: Tanksley SD, Orton TJ (eds) Isozymes in plant genetics and breeding, pt A, Elsevier, Amsterdam, pp 469–516
Wann SR, Johnson MA, Noland TL, Carlson JA (1987) Plant Cell Reports 6: 39–42
Wochok ZS, Burleson B (1974) Physiol. Plant. 31: 73–75
Author information
Authors and Affiliations
Additional information
Communicated by H. Lörz
Rights and permissions
About this article
Cite this article
Fransz, P.F., de Ruijter, N.C.A. & Schel, J.H.N. Isozymes as biochemical and cytochemical markers in embryogenic callus cultures of maize (Zea mays L.). Plant Cell Reports 8, 67–70 (1989). https://doi.org/10.1007/BF00716840
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00716840