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Genetic transformation of willows (Salix spp.) byAgrobacterium tumefaciens

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Abstract

Anin vitro transformation method has been developed for stem explants of fast-growing willow clones (Salix spp.) usingAgrobacterium tumefaciens as a vector. Transformants obtained with the strains C58 and GV3101 (pGV3851::pLD1) were selected on hormone-free medium and on medium containing kanamycin, respectively. Transformation was confirmed by Southern blot analysis and nopaline assay. Inoculation of green-house grown plants with nopaline and octopine wildtype strains and shoot or root inducing mutant strains caused undifferentiated tumors at a frequency of 0 to 80%, depending on theSalix genotype and the bacterial strain used.

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Abbreviations

BA:

benzyladenine

2,4-D:

2,4-dichlorophenoxyacetic acid

Km:

kanamycin

NPT:

neomycin phosphotransferase

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Authors and Affiliations

  1. Department of Physiological Botany, University of Uppsala, Box 540, S-75121, Uppsala, Sweden

    Tiina Vahala, Priska Stabel & Tage Eriksson

Authors
  1. Tiina Vahala
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  2. Priska Stabel
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  3. Tage Eriksson
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Communicated by H. Lörz

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Vahala, T., Stabel, P. & Eriksson, T. Genetic transformation of willows (Salix spp.) byAgrobacterium tumefaciens . Plant Cell Reports 8, 55–58 (1989). https://doi.org/10.1007/BF00716837

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  • DOI: https://doi.org/10.1007/BF00716837

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