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Genetic transformation of grapevine cells

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Abstract

Biovar 1 strains ofAgrobacterium tumefaciens have been used to transform a cell suspension culture ofVitis vinifera cv. Cabernet Sauvignon. Cocultivation of cultures withAgrobacterium strains bearing either the cointegrate pGV3850::1103neo, or the binary vector pGA474-68, each gave rise to kanamycin resistant tissue. The stable integration and expression of the neomycin phosphotransferase gene was confirmed by Southern blotting and enzymic assay, respectively.

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Abbreviations

NPTII:

neomycin phosphotransferase II

NOS:

nopaline synthase

FSAC:

fragmented shoot apex culture

TES:

N-tris-(hydroxymethyl)methyl-2-aminoethane sulfonic acid

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Communicated by R. Beachy

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Baribault, T.J., Skene, K.G.M. & Steele Scott, N. Genetic transformation of grapevine cells. Plant Cell Reports 8, 137–140 (1989). https://doi.org/10.1007/BF00716825

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  • DOI: https://doi.org/10.1007/BF00716825

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