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Genetic transformation of grapevine cells

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Biovar 1 strains ofAgrobacterium tumefaciens have been used to transform a cell suspension culture ofVitis vinifera cv. Cabernet Sauvignon. Cocultivation of cultures withAgrobacterium strains bearing either the cointegrate pGV3850::1103neo, or the binary vector pGA474-68, each gave rise to kanamycin resistant tissue. The stable integration and expression of the neomycin phosphotransferase gene was confirmed by Southern blotting and enzymic assay, respectively.

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neomycin phosphotransferase II


nopaline synthase


fragmented shoot apex culture


N-tris-(hydroxymethyl)methyl-2-aminoethane sulfonic acid


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Communicated by R. Beachy

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Baribault, T.J., Skene, K.G.M. & Steele Scott, N. Genetic transformation of grapevine cells. Plant Cell Reports 8, 137–140 (1989).

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