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The use of in vitro-grown microtuber discs inAgrobacterium-mediated transformation of Russet Burbank and Lemhi Russet potatoes

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Abstract

We have usedin vitro-grown microtuber discs in the transformation of Russet Burbank and Lemhi Russet potato (Solanum tuberosum L.) cultivars byAgrobacterium-mediated gene transfer. Transformed plants were selected by their resistance to kanamycin and identified by β-glucuronidase activity. Northern blot analysis confirmed the presence of the corresponding messenger RNA. The ability to transform these two cultivars promises significant improvements to agronomically important varieties.

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Abbreviations

MS:

Murashige and Skoog

LB:

Luria-Bertani

KS:

high kanamycin selection medium

NAA:

naphthaleneacetic acid

BA:

benzyladenine

GUS:

β-glucuronidase

EDTA:

ethylenediaminetetraacetic acid

MUG:

4-methylumbelliferyl glucuronide

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Authors and Affiliations

  1. U.S. Department of Agriculture, Agricultural Research Service, Western Regional Research Center, 800 Buchanan Street, 94710, Albany, CA, USA

    Betty K. Ishida, Gordon W. Snyder Jr. & William R. Belknap

Authors
  1. Betty K. Ishida
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  2. Gordon W. Snyder Jr.
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  3. William R. Belknap
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Communicated by J.M. Widholm

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Ishida, B.K., Snyder, G.W. & Belknap, W.R. The use of in vitro-grown microtuber discs inAgrobacterium-mediated transformation of Russet Burbank and Lemhi Russet potatoes. Plant Cell Reports 8, 325–328 (1989). https://doi.org/10.1007/BF00716665

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  • DOI: https://doi.org/10.1007/BF00716665

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