Summary
Plants were regenerated from maize (Zea mays L.) protoplasts isolated from embryogenic cell suspensions. The donor maize suspension cultures were established from friable callus initiated from microspores of a commercial supersweet hybrid (sh2sh2). The frequency of cell colony formation was higher when protoplasts were cultured on feeder layers of maize cells as compared with a liquid thin layer method. It was demonstrated that haploid and dihaploid soil-grown plants can be regenerated from maize protoplasts isolated from haploid cell cultures.
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References
Abdullah R, Cocking EC, Thompson JA (1986) Efficient plant regeneration from rice protoplasts through somatic embryogenesis. Bio/Technology 4:1087–1090
Cai QG, Kuo CS, Qian YQ, Jing RX, Zhou YL (1987) Plant regeneration from protoplasts of corn (Zea mays L). Acta Bot Sinica 29:453–458
Chourey PS, Zurawski DB (1981) Callus formation from protoplasts of a maize cell culture. Theor Appl Genet 59:341–344
Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY, Bi FY (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci Sinica 18:659–668
Dalton SJ (1988) Plant regeneration from cell suspension protoplasts ofFestuca arundinacea Schreb. (tall fescue) andLolium perenne L. (perennial ryegrass). J Plant Physiol 132:170–175
Davey MR, Power JB (1988) Aspects of protoplast culture and plant regeneration. Plant Cell Tissue Organ Cult 12:115–125
Earle ED, Gracen VE (1985) Somaclonal variation in progeny of plants from corn tissue cultures. In: Henke RR, Hughes KW, Constantin MJ, Hollaender A (eds) Tissue Culture in Forestry and Agriculture, Plenum Press New York London, pp 139–151
Edallo S, Zucchinalli C, Perezin M, Salamini F (1981) Chromosomal variation and frequency of spontaneous mutation associated with in vitro culture and plant regeneration in maize. Maydica 26:39–56
Gu MG, Zhang XQ (1982) Differentiation potential and chromosome stability of pollen callus of maize in subcultures. Acta Bot Sinica 24:319–325
Horn ME, Conger BV, Harms CT (1988) Plant regeneration from protoplasts of embryogenic suspension cultures of orchardgrass (Dactylis glomerata L.). Plant Cell Reports 7:371–374
Imbrie-Milligan CW, Kamo KK, Hdges TK (1987) Microacllus growth from maize protoplasts. Planta 171:58–64
Kao KN, Michayluk MR (1975) Nitritional requirements for growth ofVicia hajastana cells and protoplasts at very low population density in liquid media. Planta 126:105–110
Kamo KK, Chang KL, Lynn ME, Hodges TK (1987) Embryogenic callus formation from maize protoplasts. Planta 172:245–251
Kyozuka J, Hayashi Y, Shimamoto K (1987) High frequency plant regeneration from rice protoplasts by novel nurse culture methods. Mol Gen Genet 206:408–413
Ludwig SR, Somers DA, Petersen WL, Pohlman RF, Zarowits MA, Gengenbach BG, Messing J (1985) High frequency callus formation for maize protoplasts. Theor Appl Genet 71:344–350
McCoy TJ, Phillips RL (1982) Chromosome stability in maize (Zea mays) tissue cultures and sectoring in some regenerated plants. Can J Genet Cytol 24:559–565
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Ogura H, Kyozuka J, Hayashi Y, Koba T, Shimamoto K (1987) Field performance and cytology of protoplast-derived rice (Oryza sativa): high yield and low degree of variation of four Japonica cultivars. Theor Appl Genet 74:670–676
Potrykus I, Harms CT, Lörz H, Thomas E (1977) Callus formation from stem protoplasts of corn (Zea mays L.). Mol Gen Genet 156:347–350
Potrykus I, Harms CT, Lörz H (1979) Callus formation from cell culture protoplásts of corn (Zea mays L.). Theor Appl Genet 54:209–214
Prioli LM, Söndahl MR (1989) Plant regeneration and recovery of fertile plants from protoplasts of maize. Bio/Technology 7:589–594
Rhodes CA, Lowe KS, Ruby KL (1988) Plant regeneration from protoplasts isolated from embryogenic maize cell cultures. Bio/Technology 6:56–60
Shillito RD, Carswell GK, Johnson CM, DiMaio J, Harms CT (1989) Regeneration of fertile plants from protoplasts of elite inbred maize. Bio/Technology 7:581–587
Srinivasan C, Vasil IK (1986) Plant regeneration from protoplasts of sugarcane (Saccharum officinarum L.). J Plant Physiol 126:41–48
Toriyama K, Hinata K, Sasaki T (1986) Haploid and diploid plant regeneration from protoplasts of anther callus in rice. Theor Appl Genet 73:16–19
Vasil V, Vasil IK (1987) Formation of callus and somatic embryos from protoplasts of a commercial hybrid of maize (Zea mays L.). Theor Appl Genet 73:793–798
Yamada Y, Zhi-Qi Y, Ding-Tai T (1986) Plant regeneration from protoplast-derived callus of rice (Oryza sativa L.). Plant Cell Rep 5:85–88
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Communicated by C. T. Harms
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Sun, C.S., Prioli, L.M. & Söndahl, M.R. Regeneration of haploid and dihaploid plants from protoplasts of supersweet (sh2sh2) corn. Plant Cell Reports 8, 313–316 (1989). https://doi.org/10.1007/BF00716662
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DOI: https://doi.org/10.1007/BF00716662