Virchows Archiv A

, Volume 412, Issue 2, pp 161–168 | Cite as

The extracellular matrix in oral Kaposi sarcoma (AIDS): the immunohistochemical distribution of collagens type IV, V, VI, of procollagens type I and III, of laminin and of undulin

  • Jürgen Becker
  • Detlef Schuppan
  • Peter Reichart


Twelve oral AIDS-associated Kaposi sarcomas (KS) were studied for the distribution of extracellular matrix components using indirect immunofluorescence. Staining for basement membrane (BM) components revealed two distinct patterns of distribution: a delicate and partly fragmented lining of BMs around sinusoid-like vascular spaces or an occasional diffuse interstitial fluorescence in the tumour stroma; or an irregular broad rim of fluorescence in walls of larger blood vessels. These findings support a vascular cell origin of the endothelial- and spindle cell component in KS. The tumour stroma was almost completely negative for collagen type V and undulin, whereas an intensive fluorescence was noted for procollagens type I, III and collagen type VI. In areas adjacent to KS a loss of procollagens type I and III, collagens type V, VI and undulin was noted. An intimal sheath of collagen type V was usually absent from blood vessels of the tumour or the peritumourous connective tissue. Immunohistochemical findings indicate that the preexisting interstitial connective tissue matrix is destroyed during tumour invasion and that subsequently procollagens type I, III and collagen type VI are synthesized de novo by cells of the tumour stroma.

Key words

AIDS Kaposi sarcoma Collagen Extracellular matrix Basement membrane 


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  1. Becker J, Schuppan D, Hahn EG, Albert G, Reichart P (1986a) The immunohistochemical distribution of collagens type IV, V, VI and of laminin in the human oral mucosa. Arch Oral Biol 31:179–186Google Scholar
  2. Becker J, Schuppan D, Benzian H, Bals T, Hahn EG, Cantaluppi C, Reichart P (1986b) Immunohistochemical distribution of collagen types IV, V and VI and of procollagens types I and III in human alveolar bone and dentine. J Histochem Cytochem 34:1417–1429Google Scholar
  3. Becker J, Schuppan D, Reichart P (1987) Immunhistochemische Untersuchungen zum Gefäßwandaufbau in peripheren Riesenzellgranulomen. 10. Jahrestagung des Arbeitskreises Oralpathologie, Heidelberg, 02. 06. 1987Google Scholar
  4. Bendelac A, Kanitakis J, Chouvet B, Thiovolet J (1985) Basement membranes in Kaposi's sarcoma: An immunohistochemical and ultrastructural study. Pathol Res Pract 180:626–632Google Scholar
  5. Bornstein P, Sage H (1980) Structurally distinct collagen types. Annu Rev Biochem 49:957–1003Google Scholar
  6. Bruns RR, Press W, Engvall E, Timpl R, Gross J (1986) Type VI collagen in extracellular, 100 nm periodic filaments and fibrils: Identification by immunoelectron microscopy. J Cell Biol 103:393–404Google Scholar
  7. Friedman-Kien AE (1984) Kaposi's Sarcoma: An opportunistic neoplasm. J Invest Dermatol 82:446–448Google Scholar
  8. Green TL, Beckstead JH, Lozada-Nur F, Silverman S, Hansen LS (1984) Histopathologic spectrum of oral Kaposi's sarcoma. Oral Surg 58:306–314Google Scholar
  9. Knowles DM, Tolidijan B, Marboe C, D'Agati V, Grimes M, Chess L (1984) Monoclonal anti-human monocyte anti-bodies OKM1 and OKM5 possess distinctive tissue distribution including differential reactivity with vascular endothelium. J Immunol 132:2170–2173Google Scholar
  10. Kramer RH, Gia-Miin F, Hwang CBC, Conant MA, Greenspan JS (1985) Basement membrane and connective tissue proteins in early lesions of Kaposi's sarcoma associated with AIDS. J Invest Dermatol 84:516–520Google Scholar
  11. Langford-Kuntz A, Gelderblom HR, Winkel T, Reichart P (1987) Ultrastructural findings in oral Kaposi Sarcoma (AIDS). J Oral Pathol (in press)Google Scholar
  12. Leu HL, Odermatt B (1985) Multicentric angiosarcoma (Kaposi's sarcoma). Virchows Arch [A] 408:29–41Google Scholar
  13. Mc Nutt NS, Van Fletscher, Conant MA (1983) Early lesions of Kaposi's sarcoma in homosexual men. An ultrastructurat comparison with other vascular proliferations in skin. Am J Pathol 111:62–77Google Scholar
  14. Millard PR, Heryet AR (1985) An immunohistological study of factor VIII related antigen and Kaposi's sarcoma using polyclonal and monoclonal antibodies. J Pathol 146:31–38Google Scholar
  15. Murray JF, Lothe F (1962) The histopathology of Kaposi's sarcoma. Acta Un Int Cancer 18:413–428Google Scholar
  16. O'Connell KM (1977) Kaposi's sarcoma: histopathological study of 159 cases from Malawi. J Clin Pathol 30:687–695Google Scholar
  17. Reichart PA, Gelderblom HR, Becker J, Kuntz A (1987) The HIV infection: Virology, etiology, origin, immunology, precautions and clinical observations in 110 patients. Int J Oral Maxillofacial Surg 16:129–153Google Scholar
  18. Risteli J, Bächinger HP, Engel J, Furthmayr H, Timpl R (1980) 7-S collagen: characterization of an unusual basement membrane structure. Eur J Biochem 108:239–250Google Scholar
  19. Risteli J, Timpl R (1981) Isolation and characterization of pepsin fragments of laminin from human placental and renal basement membranes. Biochem J 193:749–755Google Scholar
  20. Rutgers JL, Wieczorek R, Bonetti F, Kaplan KL, Posnett DN, Friedman-Kien AE, Knowles DM (1986) The expression of endothelial cell surface antigens by AIDS-associated Kaposi's sarcoma. Am J Pathol 122:493–499Google Scholar
  21. Sage H, Pritzl P, Bornstein P (1981) Secretory phenotypes of endothelial cells in culture: Comparison of aortic, venous, capillary, and corneal endothelium. Arteriosclerosis 1:427–442Google Scholar
  22. Schuppan D, Hahn EG (1987) Components of the extracellular matrix (collagens, glycoproteins, elastin, proteoglycans): In: Wolf JR (ed) Mesenchymal epithelial interactions in neural development NATO ASI series. Springer, Berlin Heidelberg New York, pp 1–27Google Scholar
  23. Schuppan D, Rühlmann T, Hahn EG (1985) Radioimmunoassay for human type VI collagen and its application to tissue and body fluids. Anal Biochem 149:238–247Google Scholar
  24. Schuppan D, Becker J, Böhm H, Hahn EG (1986a) Immunofluorescent localization of type V collagen as a fibrillar component of the interstitial connective tissue of human oral mucosa, artery and liver. Cell Tissue Res 243:535–543Google Scholar
  25. Schuppan D, Besser M, Schwarting R, Hahn EG (1986b) Radioimmunoassay for the carboxy-terminal cross-linking domain of type IV (basement membrane) procollagen in body fluids. J Clin Invest 78:241–248Google Scholar
  26. Schuppan D, Cantaluppi C, Schuppan F, Becker J, Fleischmajer R, Hahn EG (1987) Undulin, a type I collagen associated glycoprotein from connective tissue. (submitted for publication)Google Scholar
  27. Timpl R, Wiedemann H, Delden V van, Furthmayr H, Kühn K (1981) A network model for the organization of type IV collagen molecules in basement membranes. Eur J Biochem 120:203–211Google Scholar
  28. Vaes G (1985) Macrophage secretory products and connective tissue remodeling: role of macrophage enzymes and of-matrix regulatory monokines. In: Dean RT, Stahl P (eds) Developments in cell biology. Butterworths, London, pp 99–117Google Scholar
  29. Von der Mark H, Aumailley M, Wick G, Fleischmajer R, Timpl R (1984) Immunochemistry, genuine size and tissue localization of collagen type VI. Eur J Biochem 142:493–502Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Jürgen Becker
    • 1
  • Detlef Schuppan
    • 2
  • Peter Reichart
    • 1
  1. 1.Department of Oral Surgery, Faculty of DentistryFree University of BerlinBerlin 65
  2. 2.Steglitz Medical Center, Department of MedicineFree University of BerlinBerlin 41

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