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The reductive enzymatic cleavage of thiosulfate

Methods and application

Summary

Methods presently used for the enzymatic assay of the thiosulfate cleaving reaction in bacterial cell-free systems are critically examined. Conditions under which strong acids are used to terminate the reaction and to release H2S proved to be unsuitable. A non-enzymatic production of H2S under such conditions is demonstrated. A reliable procedure for the measurement of H2S production from the enzymatic cleavage of thiosulfate is described. This method was used to measure the thiosulfate cleaving reaction catalyzed by cell-free extracts of phototrophic bacteria of the genusChromatium. As reductants, hydrogen-hydrogenase/methyl viologen system, reduced glutathione or dihydrolipoate were used. The same extract fraction catalyzed the rhodanese reaction.

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References

  1. Beisenherz, G., Boltze, H. J., Bücher, T., Czok, R., Garbade, K. H., Meyer-Arendt, E., Pfleiderer, G.: Diphosphofructose-Aldolase, Phosphoglycerinaldehyde-Dehydrogenase, Michsäuredehydrogenase, Glycerophosphat-Dehydrogenase und Pyruvat-Kinase aus Kaninchenmuskel in einem Arbeitsgang. Z. Naturforsch.8b, 555–577 (1953).

    Google Scholar 

  2. Bowen, T. J., Butler, P. J., Happold, F. C.: Some properties of the rhodanese system ofThiobacillus denetrificans. Biochem. J.97, 651–657 (1965).

    Google Scholar 

  3. De Groot, G. N., Stouthammer, A. H.: Regulation of reductase formation inProteus mirabilis. I. Formation of reductases and enzymes of the formic hydrogenylase complex in the wild type and in chlorate-resistant mutants. Arch. Mikrobiol.66, 220–223 (1969).

    Google Scholar 

  4. Haschke, R., Campbell, L. L.: Thiosulfate reductase ofDesulfovibrio vulgaris. J. Bact..106, 603–607 (1971).

    Google Scholar 

  5. Kaji, A., McElroy, W. D.: Mechanism of H2S formation from thiosulfate. J. Bact.77, 630–637 (1959).

    Google Scholar 

  6. Koj, A., Frendo, J.: Oxidation of thiosulfate to sulfate in animal tissues. Folia biol. (Praha)15, 49–63 (1967).

    Google Scholar 

  7. Much, A. M., Hurlbert, R. E.: Characterization of thiosulfate reductase ofChromatium strain D. Bact. Proc.1971, 149.

  8. Nakatsukasa, W., Akagi, J. M.: Thiosulfate reductase iolated fromDesulfotomaculum nigrificans. J. Bact.98, 429–433 (1969).

    Google Scholar 

  9. Pachmayr, F.: Vorkommen und Bestimmung von Schwefelverbindungen in Mineralwasser. Diss., München 1960.

  10. Pfennig, N., Lippert, K. D.: Über das Vitamin B12-Bedürfnis phototropher Schwefelbakterien. Arch. Mikroiol.55, 245–256 (1966).

    Google Scholar 

  11. Pichinoty, F.: Répression par l'oxygène de la biosynthèse de la thiosulfateréductase deProteus vulgaris. Experientia (Basel)18, 501–503 (1962).

    Google Scholar 

  12. Roy, A. B., Trudinger, P. A.: In: The biochemistry of inorganic compounds of sulfur, pp. 190–204. Cambridge: Cambridge University Press 1970.

    Google Scholar 

  13. Smith, A. J., Lascelles, J.: Thiosulfate metabolism and rhodanese inChromatium sp. strain D. J. gen. Microbiol.42, 357–370 (1966).

    Google Scholar 

  14. Sörbo, B. H.: Rhodanese. In: Methods enzymology II (S. Colowick and N. Kaplan, Eds.) p. 334. New York: Acad. Press 1955.

    Google Scholar 

  15. Strauss, G., Nickerson, W. J.: Photochemical cleavage of H2O by Riboflavin. J. Amer. chem. Soc.83, 8187–8191 (1961).

    Google Scholar 

  16. Suh, B., Akagi, J. M.: Formation of thiosulfate from sulfite byDesulfovibrio vulgaris. J. Bact.99, 210–215 (1969).

    Google Scholar 

  17. Trüper, H. G., Pfennig, N.: Sulfur metabolism in thiorhodaceae. III. Storage and turnover of thiosulfate sulfur inThiocapsa floridana andChromatium species. Antonie v. Leeuwenhoek32, 261–276 (1966).

    Google Scholar 

  18. Van, Niel, C. B.: In: Bergey's manual of determinative bacteriology, 7th ed. (R. S. Breed, E. G. D. Murray, and N. R. Smith, Eds.), pp. 38–61. Baltimore: Williams and Wilkins 1957.

    Google Scholar 

  19. Villarejo, M., Westley, J.: Mechanism of rhodanese catalysis of thiosulfate-lipoate oxidation-reduction. J. biol. Chem.238, 4016–4020 (1963).

    Google Scholar 

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Hashwa, F., Pfennig, N. The reductive enzymatic cleavage of thiosulfate. Archiv. Mikrobiol. 81, 36–44 (1972). https://doi.org/10.1007/BF00715022

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Keywords

  • Glutathione
  • Enzymatic Assay
  • Thiosulfate
  • Strong Acid
  • Reduce Glutathione