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Effects of temperature on the conformation of the endoplasmic reticulum and on starch accumulation in leaves with the symplasmic minor-vein configuration

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Abstract

The phloem-loading-related effects of temperature on leaf ultrastructure were studied in seven species having numerous plasmodesmatal connections between the mesophyll and phloem (symplasmic minor-vein configuration). The response to temperature (between 5 and 30 °C) was characterized by drastic changes in the endoplasmic-reticulum labyrinth (ER labyrinth) of intermediary cells, in the position of the vacuole in bundle-sheath cells, and in the starch content in the chloroplasts of bundle-sheath cells and mesophyll cells. At temperatures above 20 °C, the ER system in the intermediary cells reached its maximal volume, while the vacuole in bundlesheath cells was positioned centripetally (proximal to the intermediary cell). With decreasing temperature, the ER labyrinth in intermediary cells gradually contracted till the ER was fully collapsed at 10 °C and the vacuole in bundle-sheath cells moved to a more centrifugal position. The apparent elimination of photosynthate transport via the ER and plasmodesmata at temperatures lower than 10 °C led to starch accumulation in the chloroplasts of bundle-sheath cells and mesophyll cells. All of these changes were fully temperature-reversible and probably reflect changes in the balance between photosynthate transport and storage. The ultrastructural shifts appear to be correlated with the passage of photosynthate through the intermediary cells and, as a consequence, with the rate of phloem loading at various temperatures. A contraction of the ER/plasmodesmata system imposed by cytoskeletal reorganisation is discussed as the reason for the blockage of phloem loading at low temperatures in association with the general chilling sensitivity of these species.

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Abbreviations

BSC:

bundle-sheath cell

IC:

intermediary cell

MC:

mesophyll cell

PD:

plasmodesmata

PFD:

photon flux density

SE/CC-complex:

sieve element/companion cell complex

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Authors and Affiliations

  1. Department of Ecophysiology, Komarov Botanical Institute, Professor Popovstreet 2, 197376, St. Petersburg, Russia

    Yuri V. Gamalei, Marina V. Pakhomova & Anna V. Sjutkina

  2. Transport Physiology Research Group, Department of Plant Ecology and Evolutionary Biology, University of Utrecht, Sorbonnelaan 16, 3584, Utrecht, CA, The Netherlands

    Aart J. E. van Bel

Authors
  1. Yuri V. Gamalei
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  2. Aart J. E. van Bel
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  3. Marina V. Pakhomova
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  4. Anna V. Sjutkina
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The authors gratefully acknowledge the financial support by NWO (Dutch Organization for Scientific Research).

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Gamalei, Y.V., van Bel, A.J.E., Pakhomova, M.V. et al. Effects of temperature on the conformation of the endoplasmic reticulum and on starch accumulation in leaves with the symplasmic minor-vein configuration. Planta 194, 443–453 (1994). https://doi.org/10.1007/BF00714455

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  • DOI: https://doi.org/10.1007/BF00714455

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