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Effect of interleukin-1 receptor antagonist (IL-1RA) on histamine and serotonin release by rat basophilic leukemia cells (RBL-2H3) and peritoneal mast cells

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Abstract

Recently, it has been appreciated that cultured mast cells are significant sources of cytokines. However, the role of interkeukin-1 (IL-1) on mast cells and/or basophil degranulation is still unclear. In this report we provide evidence that rat basophilic leukemia cells (RBLC) cultured with a natural inhibitor of IL-1, interleukin-1 receptor antagonist (IL-1RA) (500 ng/ml) for 48 h, strongly inhibited the spontaneous release of serotonin (5HT) and histamine (from 22.50 to 43.49%), compared to untreated cells (control). When IL-1RA-treated and untreated RBLC were stimulated with a secretagogue (anti-IgE), no difference was found in the percent of 5HT and histamine release. Moreover, in another set of experiments using rat peritoneal mast cells (RPMC) treated and untreated with IL-1RA, we found that IL-1RA did not affect the release of 5HT or histamine, even when the secretagogue anti-IgE or compound 48/80 (C48/80) were used. The present studies describe an additional biological activity of IL-1RA, inhibiting histamine and 5HT release from RBLC cultures.

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Abbreviations

IL-1:

interleukin-1

RA:

receptor antagonist

5HT:

serotonin

RBLC:

rat basophilic leukemia cells

RPMC:

rat peritoneal mast cells

IgE:

immunoglobulin E

FcΣ :

immunoglobulin E receptor

CPM:

counts per minute

BSA:

bovine serum albumin

C48/80:

compound 48/80

TNF:

tumor necrosis factor

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Authors and Affiliations

  1. Immunology Division, Institute of Experimental Medicine, University of Chieti, Via dei Vestini, 66100, Chieti, Italy

    Pio Conti, Renato C. Barbacane & Marcella Reale

  2. Dermatology Division, University of Chieti, Via dei Vestini, 66100, Chieti, Italy

    Claudio Feliciani & Sandra Mammarella

  3. Department of Pharmacology and Experimental Therapeutics, Tufts University School of Medicine, 136 Harrison Avenue, 02111, Boston, Massachusetts, USA

    William Bouchet, Leena Kudchadker, Iman Haggag & Theoharis C. Theoharides

  4. Laboratoire de Biochimie Cellulaire, College de France, Paris, France

    Gilles Bruneau & Jean Thibault

Authors
  1. Pio Conti
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  2. William Bouchet
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  3. Claudio Feliciani
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  4. Sandra Mammarella
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  5. Leena Kudchadker
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  6. Renato C. Barbacane
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  7. Marcella Reale
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  9. Gilles Bruneau
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  11. Theoharis C. Theoharides
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Conti, P., Bouchet, W., Feliciani, C. et al. Effect of interleukin-1 receptor antagonist (IL-1RA) on histamine and serotonin release by rat basophilic leukemia cells (RBL-2H3) and peritoneal mast cells. Mol Cell Biochem 155, 61–68 (1996). https://doi.org/10.1007/BF00714334

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  • DOI: https://doi.org/10.1007/BF00714334

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