Abstract
Using two-dimensional polyacrylamide gel electrophoresis, the GroEL homologue ofBacillus subtilis was shown to be induced upon infection with Ø105clz, a clear plaque mutant of the temperate bacteriophage Ø105. Western blotting of one dimensional polyacrylamide gels also showed the induction of the GroEL homologue when cells were infected with Ø105clz.
This is a preview of subscription content, log in via an institution to check access.
References
Arnosti DN, Singer VL & Chamberlin MJ (1986) Characterization of heat shock inBacillus subtilis. J. Bacteriol. 168: 1243–1249
Brissette JL, Russel M, Weiner L & Model P (1990) Phage shock protein, a stress protein ofEscherichia coli. Proc. Natl. Acad. Sci. USA 87: 862–866
Brissette JL, Weiner L, Ripmaster TL & Model P (1991) Characterization and sequence of theEscherichia coli stress-inducedpsp operon. J. Mol. Biol. 220: 35–48
Carrascosa JL, Garcia JA & Salas M (1982) A protein similar toEscherichia coli groEL is present inBacillus subtilis. J. Mol. Biol. 158: 731–737
Drahos DJ & Hendrix RW (1982) Effect of bacteriophage lambda infection on synthesis ofgroE protein and otherEscherichia coli proteins. J. Bacteriol. 149: 1050–1063
Friedman DI, Olson ER, Georgopoulos C, Tilly K, Herskowitz I & Banuett F (1984) Interactions of bacteriophage and host macromolecules in the growth of bacteriophage lambda. Microbiol. Rev. 48: 299–325
Kochan J & Murialdo H (1982) Stimulation ofgroE synthesis inEscherichia coli by bacteriophage lambda infection. J. Bacteriol. 149: 1166–1170
Miller BS, Kennedy TE & Streips UN (1991) Molecular characterization of specific heat shock proteins inBacillus subtilis. Curr. Micro. 22: 231–236
Neidhardt FC & VanBogelen RA (1987) Heat shock response. In: Neidhardt FC, Ingraham JL, Magasanik B, Low KB, Schaechter M & Umbarger HE (Eds)Escherichia coli andSalmonella typhimurium: Cellular and Molecular Biology, vol 2 (pp 1334–1345). Washington, DC: American Society for Microbiology
Spizizen J (1958) Transformation of biochemically deficient strains ofBacillus subtilis by deoxyribonucleate. Proc. Natl. Acad. Sci. USA 44: 1072–1078
Streips UN & Polio FW (1985) Heat shock proteins in bacilli. J. Bacteriol. 162: 434–437
Young KD, Anderson RJ & Hafner RJ (1989) Lysis ofEscherichia coli by the bacteriophage ØX174 E protein: Inhibition of lysis by heat shock proteins. J. Bacteriol. 171: 4334–4341
Zylicz M, Ang D, Liberek K & Georgopoulos C (1989) Initiation of lambda DNA replication with purified host-and bacteriophage-encoded proteins: the role of the DnaK, DnaJ, and GrpE heat shock proteins. EMBO J. 8: 1601–1608
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Staples, R.R., Miller, B.S. & Streips, U.N. Bacteriophage Ø105clz induces the GroEL-homologue protein inBacillus subtilis . Antonie van Leeuwenhoek 61, 339–342 (1992). https://doi.org/10.1007/BF00713942
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00713942