Abstract
We performed experiments in parallel to study the rate of synthesis of cell wall polysaccharides and the activity of glycosyl transferases inSaccharomyces cerevisiae after arrest of acdc 28 mutant in G1 phase by either addition of alpha-factor or transfer to the non-permissive temperature. Both effectors brought about similar time-dependent increases in the rate of synthesis and deposition of the cell wall polysaccharides chitin, glucan and mannan. These changes in cell wall composition were accompanied by an increase in the specific activities of glucan and chitin synthetases. This increase was inhibited by cycloheximide suggesting that it representedde novo enzyme biosynthesis and not enzyme activation. Our data are consistent with the notion that both alpha-factor and thecdc 28 mutation affect the same stage-specific function that controls the temporal expression of glycosyl transferases.
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Abbreviations
- GlcNAc:
-
N-acetyl glucosamine
- UDPGIcNAc:
-
uridine-diphosphate-N-acetyl glucosamine
- UDPGlc:
-
uridine-diphosphate glucose
- TCA:
-
trichloroacetic acid
- EDTA:
-
ethylene diamino tetraacetate
- TAME:
-
tosyl-L-arginyl methyl ester
- GTP:
-
guanosine triphosphate
- WGA:
-
wheat germ agglutinin
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Díaz, S., Zínker, S. & Ruiz-Herrera, J. Alterations in the cell wall ofSaccharomyces cerevisiae induced by the alpha sex factor or a mutation in the cell cycle. Antonie van Leeuwenhoek 61, 269–276 (1992). https://doi.org/10.1007/BF00713935
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DOI: https://doi.org/10.1007/BF00713935