Acta Neuropathologica

, Volume 83, Issue 4, pp 408–414 | Cite as

Immunocytochemical and ultrastructural studies of eosinophilic granular bodies in astrocytic tumors

  • S. Murayama
  • T. W. Bouldin
  • K. Suzuki
Regular Papers


Eosinophilic granular bodies (EGBs) are studied immunocytochemically and ultrastructurally in a case of low-grade and a case of high-grade astrocytoma. EGBs are recognized as brightly eosinophilic round bodies of variable size in hematoxylin and eosin-stained sections. Immunocytochemically some EGBs are positive for antibodies raised against αB-crystallin, ubiquitin and glial fibrillary acidic protein with the staining patterns for each being different from one another. Ultrastructurally EGBs consist of membrane-bound round body of various diameter ranging from 50 nm to 20 μm. Small EGBs contain electron-dense homogeneous material with occasional myelin figures, while large EGBs contain small EGB-like structures within electron-dense homogeneous material or loose granular profiles. Our studies demonstrate (1) ultrastructural variety of EGB; (2) and αB-crystallin epitope in EGB; and (3) the presence of EGB in high-grade as well as low-grade astrocytoma.

Key words

Eosinophilic granular body Astrocytoma Ultrastructure Immunocytochemistry αB-crystallin 


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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • S. Murayama
    • 1
  • T. W. Bouldin
    • 1
  • K. Suzuki
    • 1
  1. 1.Department of Pathology, Division of NeuropathologyUniversity of North Carolina at Chapel HillChapel HillUSA

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