Summary
The myosin light chains of slow muscles from different species have been examined with two-dimensional gel electrophoresis. While the myosin light chain 2S of mammalian soleus muscles (rabbit, rat and guinea-pig) could not be distinguished from that of avian anterior latissimus dorsi (chicken and turkey), the 1S light chain complement of myosins shows inter- and intraspecific differences. The 1S light chain varies between birds and mammals. The 1'S light chain is absent in avian slow myosins and has an electrophoretic mobility peculiar to each mammalian species. Furthermore the relative amount of 1'S and 1S light chains varies in different muscles of the same mammalian species and among species.
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ARNDT, I. & PEPE, F. A. (1975) Antigenic specificity of red and white muscle myosin.J. Histochem. Cytochem. 23, 159–68.
BARANY, M. (1967) ATPase activity of myosin correlated with speed of muscle shortening.J. gen. Physiol. 50, 197–218.
BARANY, M. & CLOSE, R. I. (1971) The transformation of myosin in crossinnervated rat muscles.J. Physiol. 213, 455–74.
CARRARO, U., CATANI, C. & BIRAL, D. (1979) Selective maintenance of neurotrophically regulated proteins in denervated rat diaphragm.Expl Neurol. 63, 468–75.
CARRARO, U., CATANI, C. & DALLA LIBERA, L. (1981) Myosin light and heavy chains in rat gastrocnemius and diaphragm muscles after chronic denervation or reinnervation.Expl Neurol. 72, 401–12.
CARRARO, U., DALLA LIBERA, L. & SARTORE, S. (1980) Heavy and light chains of the myosins from different slow muscles.J. Musc. Res. Cell Motil. 1, 234–5.
CLOSE, R. I. (1972) Dynamic properties of mammalian skeletal muscles.Physiol. Rev. 52, 129–97.
DALLA LIBERA, L., MARGRETH, A., MUSSINI, I., CERRI, C. & SCARLATO, G. (1978) Myosin polymorphism in human skeletal muscle.Muscle and Nerve 1, 280–91.
DALLA LIBERA, L., SARTORE, S. & SCHIAFFINO, S. (1979) Comparative analysis of chicken atrial and ventricular myosin.Biochim. Biophys. Acta 581, 283–94.
DALLA LIBERA, L., SARTORE, S., PIEROBON-BORMIOLI, S. & SCHIAFFINO, S. (1980) Fast-white and fast-red isomyosins in guinea pig muscles.Biochem. biophys. Res. Commun. 96, 1662–70.
FREARSON, N. & PERRY, S. V. (1975) Phosphorylation of the light chain components from cardiac and red skeletal muscles.Biochem. J. 151, 99–107.
GAUTHIER, G. F. & PADYKULA, H. A. (1966) Cytological studies of fiber types in skeletal muscle. A comparative study of the mammalian diaphragm.J. Cell Biol. 28, 333–54.
GUTMANN, E., MELICHNA, J. & SYROVY, I. (1974) Developmental changes in contraction time, myosin properties and fibre pattern of fast and slow skeletal muscles.Physiol. Bohemoslov. 23, 19–27.
KUGELBERG, E. (1976) Histochemical composition, contraction speed and fatiguability of rat soleus motor units.J. Neurol. Sci. 27, 269–89.
LAEMMLI, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4.Nature 227, 680–5.
LOWEY, S. & RISBY, D. (1971) Light chains from fast and slow muscle myosins.Nature 234, 81–5.
MANNHERZ, H. G., & GOODY, R. S. (1976) Proteins of contractile systems.Ann. Rev. Biochem. 45, 427–65.
MARGRETH, A., SALVIATI, G., DALLA LIBERA, L., BETTO, R., BIRAL, D. & SALVATORI, S. (1980) Transition in membrane macromolecular composition and myosin isozymes during development of fast-twitch and slow-twitch muscles. InPlasticity of Muscle (edited by PETTE. D.), pp. 193–208. Berlin, New York: de Gruyter.
O'FARRELL, P. H. (1975) High resolution two-dimensional electrophoresis of proteins.J. biol. Chem. 250, 4007–21.
PETTE, D., MULLER, W., LEISNER, E. & VRBOVA, G. (1976) Time dependent effects on contractile properties, fibre population, myosin light chains, and enzymes of energy metabolism in intermittently and continuously stimulated fast twitch muscles of the rabbit.Pflügers Arch. 364, 103–12.
PIEROBON-BORMIOLI, S., SARTORE, S., VITADELLO, M. & SCHIAFFINO, S. (1980) ‘Slow’ myosins in vertebrate skeletal muscle. An immunofluorescence study.J. Cell Biol. 85, 672–81.
SCHAUB, M. C. & PERRY, S. V. (1971) The regulatory proteins of the myofibril. Characterisation and properties of the inhibitory factor (troponin B).Biochem. J. 123, 367–77.
SRETER, F. A., BALINT, M. & GERGELY, J. (1975) Structural and functional changes of myosin during development. Comparison with adult fast, slow and cardiac myosin.Devl Biol. 46, 317–25.
STEINBACH, J. H., SCHUBERT, D. & ELDRIDGE, L. (1980) Changes in cat muscle contractile proteins after prolonged muscle inactivity.Expl Neurol. 67, 655–69.
WEBER, K. & OSBORN, M. (1969) The reliability of molecular weight determinations by dodecyl sulphate-polyacrylamide gel electrophoresis.J. biol. Chem. 224, 4406–12.
WEEDS, A. J. (1976) Light chains from slow-twitch muscle myosin.Eur. J. Biochem. 66, 157–73.
WHALEN, R. G., BUTLER-BROWNE, G. S. & GROS, F. (1978) Identification of a novel form of myosin light chain present in embryonic muscle tissue and cultured muscle cells.J. molec. Biol. 126, 415–31.
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Carraro, U., Libera, L.D. & Catani, C. Myosin light chains of avian and mammalian slow muscles: evidence of intraspecific polymorphism. J Muscle Res Cell Motil 2, 335–342 (1981). https://doi.org/10.1007/BF00713271
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DOI: https://doi.org/10.1007/BF00713271