Journal of Muscle Research & Cell Motility

, Volume 5, Issue 5, pp 503–513 | Cite as

The maximum velocity of shortening during the early phases of the contraction in frog single muscle fibres

  • V. Lombardi
  • G. Menchetti
Papers

Summary

The maximum velocity of shortening (Vmax) was determined at preset times during the development and the plateau of isometric tetani in single fibres isolated from the tibialis anterior muscle of the frog. Experiments were performed at low temperature (3.6–6° C) and at about 2.25 µm sarcomere length. The controlled velocity release method was used.Vmax was measured by determining the lowest velocity of release required to keep the tension at zero. Extreme care was taken in dissection and mounting of the fibres in order to make the passive series compliance very small.

The value ofVmax at the end of the latent period for the development of isometric tension (at 4.5° C about 10 ms after the beginning of the stimulus volley) was already the same as later during either the tension rise or at the plateau of isometric tetani. These results show that the value ofVmax of intact fibres is independent of time and activation subsequent to the latent period, and suggest that the cycling rate of the crossbridges may thus attain its steady-state value just at the end of the isometric latent period.

Keywords

Latent Period Maximum Velocity Single Fibre Tibialis Anterior Muscle Passive Series 

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References

  1. ABBOTT, B. C. & RITCHIE, J. M. (1951) The onset of shortening in striated muscle.J. Physiol. 113, 336–45.Google Scholar
  2. AMBROGI-LORENZINI, C., COLOMO, F. & LOMBARDI, V. (1983) Development of force-velocity relation, stiffness and isometric tension in frog single muscle fibres.J. Musc. Res. Motility 4, 177–89.Google Scholar
  3. BAYLOR, S. M., CHANDLER, W. K. & MARSHALL, M. W. (1982) Use of metallochromic dyes to measure changes in myoplasmic calcium during activity in frog skeletal muscle fibres.J. Physiol. 331, 139–77.Google Scholar
  4. CECCHI, G., COLOMO, F. & LOMBARDI, V. (1976) A loudspeaker servo system for determination of mechanical characteristics of isolated muscle fibres.Boll. Soc. ital. Biol. sper. 52, 733–6.Google Scholar
  5. CECCHI, G., COLOMO, F. & LOMBARDI, V. (1978) Force-velocity relation in normal and nitrate-treated single muscle fibres during rise of tension in an isometric tetanus.J. Physiol. 285, 257–73.Google Scholar
  6. CECCHI, G., COLOMO, F. & LOMBARDI, V. (1981) Force-velocity relation in deuterium-oxidetreated frog single muscle fibres during the rise of tension in an isometric tetanus.J. Physiol. 317, 207–21.Google Scholar
  7. EDMAN, K. A. P. (1979) The velocity of unloaded shortening and its relation to sarcomere length and isometric force in vertebrate muscle fibres.J. Physiol. 291, 143–59.Google Scholar
  8. EDMAN, K. A. P., MULIERI, L. A. & SCUBON-MULIERI, B. (1976) Non-hyperbolic force-velocity relationship in single muscle fibres.Acta physiol. scand. 98, 143–56.Google Scholar
  9. FENN, W. O. & MARSH, B. S. (1935) Muscular force at different speeds of shortening.J. Physiol. 85, 277–97.Google Scholar
  10. FORD, L. E., HUXLEY, A. F. & SIMMONS, R. M. (1977) Tension responses to sudden length change in stimulated frog muscle fibres near slack length.J. Physiol. 269, 441–515.Google Scholar
  11. GULATI, J. & BABU, A. (1983) Crossbridge properties at varied degrees of activation of isolated fibers and the mechanism of contraction with temp step.Biophys. J. 41, 35a.Google Scholar
  12. GULATI, J. & PODOLSKY, R. J. (1981) Isotonic contraction of skinned muscle fibres on a slow time base. Effects of ionic strength and calcium.J. gen. Physiol. 78, 233–57.Google Scholar
  13. HILL, A. V. (1938) The heat of shortening and the dynamic constants of muscle.Proc. R. Soc. 126, 136–95.Google Scholar
  14. HILL, A. V. (1951) The transition from rest to full activity in muscle: the velocity of shortening.Proc. R. Soc. 138, 329–38.Google Scholar
  15. HILL, D. K. (1968) Tension due to interaction between the sliding filaments in resting striated muscle. The effect of stimulation.J. Physiol. 199, 637–84.Google Scholar
  16. HUXLEY, A. F. (1957) Muscle structure and theories of contraction.Prog. Biophys. biophys. Chem. 7, 255–318.Google Scholar
  17. HUXLEY, A. F. & LOMBARDI, V. (1980) A sensitive force-transducer with resonant frequency 50 kHz.J. Physiol. 305, 15–6P.Google Scholar
  18. HUXLEY, A. F. & SIMMONS, R. M. (1971) Proposed mechanism of force generation in striated muscle.Nature 233, 533–8.Google Scholar
  19. JEWELL, B. R. & WILKIE, D. R. (1958) An analysis of mechanical components in frog's striated muscle.J. Physiol. 143, 515–40.Google Scholar
  20. JÖBSIS, F. F. & O'CONNOR, M. J. (1966) Calcium release and reabsorption in the sartorius muscle of the toad.Biochem. biophys. Res. Commun. 25, 246–52.Google Scholar
  21. JULIAN, F. J. (1971) The effect of calcium on the force-velocity relation of briefly glycerinated frog muscle fibres.J. Physiol. 218, 117–45.Google Scholar
  22. JULIAN, F. J. & MOSS, R. L. (1981) Effects of calcium and ionic strength on shortening velocity and tension development in frog skinned muscle fibres.J. Physiol. 311, 179–99.Google Scholar
  23. JULIAN, F. J. & SOLLINS, M. R. (1973) Regulation of force and speed of shortening in muscle contraction.Cold Spring Harb. Symp. quant. Biol. 37, 635–46.Google Scholar
  24. MILEDI, R., PARKER, I. & SCHALOW, G. (1977) Measurement of calcium transients in frog muscle by the use of arsenazo III.Proc. R. Soc. 198, 201–10.Google Scholar
  25. MILEDI, R., PARKER, I. & ZHU, P. H. (1982) Calcium transients evoked by action potentials in frog twitch muscle fibres.J. Physiol. 333, 655–79.Google Scholar
  26. PODOLIN, R. A. & FORD, L. E. (1983) The influence of calcium on shortening velocity of skinned frog muscle cells.J. Musc. Res. Cell Motility 4, 263–82.Google Scholar
  27. PODOLSKY, R. J. & TEICHHOLZ, L. E. (1970) The relation between calcium and contraction kinetics in skinned muscle fibres.J. Physiol. 211, 19–35.Google Scholar
  28. RÜDEL, R. & TAYLOR, S. R. (1973) Aequorin luminescence during contraction of amphibian skeletal muscle.J. Physiol. 233, 5–6P.Google Scholar
  29. SANDOW, A. & SEAMAN, T. (1964) Muscle shortening velocity in normal and potentiated contractions.Life Sci. 3, 91–6.Google Scholar
  30. STEPHENSON, D. G. S. & JULIAN, F. J. (1982) Ca++ effects on the unloaded speed of shortening (V max) of mammalian skeletal muscle fibers.Biophys. J. 37, 358a.Google Scholar
  31. TAYLOR, S. R., RÜDEL, R. & BLINKS, J. R. (1975) Calcium transients in amphibian muscle.Fed. Proc. 34, 1379–81.Google Scholar
  32. THAMES, M. D., TEICHHOLZ, L. E. & PODOLSKY, R. J. (1974) Ionic strength and the contraction kinetics of skinned muscle fibres.J. gen. Physiol. 63, 509–30.Google Scholar

Copyright information

© Chapman and Hall Ltd 1984

Authors and Affiliations

  • V. Lombardi
    • 1
  • G. Menchetti
    • 1
  1. 1.Istituto di FisiologiaUniversità di FirenzeFirenzeItaly

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