Current Genetics

, Volume 25, Issue 1, pp 73–79 | Cite as

Translation in a wheat germ cell-free system of RNA from mitochondria of the normal and Texas male-sterile cytoplasms of maize (Zea mays L.)

  • Ethan Hack
  • Carol A. Hendrick
  • Salah M. Al-Janabi
  • Virginia C. Crane
  • Lois E. Girton
Original Articles


RNA isolated from etiolated seedling shoot mitochondrial of maize (Zea mays L.) with normal (N) or Texas male-sterile (T) cytoplasm stimulated the incorporation of [35S]-methionine into protein when added to a cell-free protein-synthesizing system from wheat germ. Discrete polypeptides with molecular masses of up to approximately 67 kDa were synthesized, and the pattern of bands was distinct from that obtained with total RNA. Products of translation of T-urf13 RNA were identified by immunoprecipitation, and ofatpA, coxI, andcoxII RNA by hybrid arrest of translation by the cloned gene. several polypeptides were differentially synthesized from N and T mitochondrial RNA; these differences were more extensive than those found when isolated, intact, N and T mitochondria are allowed to synthesize proteins.

Key words

Mitochondria In-vitro translation Texas cytoplasmic male sterility T-urf13 gene 


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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Ethan Hack
    • 1
  • Carol A. Hendrick
    • 1
  • Salah M. Al-Janabi
    • 1
  • Virginia C. Crane
    • 1
  • Lois E. Girton
    • 1
  1. 1.Department of BotanyIowa State UniversityAmesUSA

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