Current Genetics

, Volume 25, Issue 1, pp 73–79 | Cite as

Translation in a wheat germ cell-free system of RNA from mitochondria of the normal and Texas male-sterile cytoplasms of maize (Zea mays L.)

  • Ethan Hack
  • Carol A. Hendrick
  • Salah M. Al-Janabi
  • Virginia C. Crane
  • Lois E. Girton
Original Articles
  • 45 Downloads

Abstract

RNA isolated from etiolated seedling shoot mitochondrial of maize (Zea mays L.) with normal (N) or Texas male-sterile (T) cytoplasm stimulated the incorporation of [35S]-methionine into protein when added to a cell-free protein-synthesizing system from wheat germ. Discrete polypeptides with molecular masses of up to approximately 67 kDa were synthesized, and the pattern of bands was distinct from that obtained with total RNA. Products of translation of T-urf13 RNA were identified by immunoprecipitation, and ofatpA, coxI, andcoxII RNA by hybrid arrest of translation by the cloned gene. several polypeptides were differentially synthesized from N and T mitochondrial RNA; these differences were more extensive than those found when isolated, intact, N and T mitochondria are allowed to synthesize proteins.

Key words

Mitochondria In-vitro translation Texas cytoplasmic male sterility T-urf13 gene 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Anderson CW, Straus JW, Dudock BS (1983) Methods Enzymol 101:635–644Google Scholar
  2. Anderson DJ, Blobel G (1983) Methods Enzymol 96:111–120Google Scholar
  3. Bégu D, Graves P-V, Domec C, Arselin G, Litvak S, Araya A (1990) Plant Cell 2:1283–1290Google Scholar
  4. Braun CJ, Levings CS III (1985) Plant Physiol 79:571–577Google Scholar
  5. Braun CJ, Siedow JN, Williams ME, Levings CS III (1989) Proc Natl Acad Sci USA 85:4435–4439Google Scholar
  6. Breiman A, Galun E (1990) Plant Sci 71:3–19Google Scholar
  7. Chomczynski P, Sacchi N (1987) Anal Biochem 162:156–159Google Scholar
  8. Covello PS, Gray MW (1989) Nature 341:662–666Google Scholar
  9. Dawson AJ, Jones VP, Leaver CJ (1984) EMBO J 3:2107–2113Google Scholar
  10. De Ronde A, Van Loon APGM, Grivell LA, Kohli J (1980) Nature 287:361–363Google Scholar
  11. Dewey RE, Levings CS III, Timothy DH (1985a) Plant Physiol 79:914–919Google Scholar
  12. Dewey RE, Schuster AM, Levings CS III, Timothy DH (1985 b) Proc Natl Acad Sci USA 82:1015–1019Google Scholar
  13. Dewey RE, Levings CS III, Timothy DH (1986) Cell 44:439–449Google Scholar
  14. Dewey RE, Timothy DH, Levings CS III, (1987) Proc Natl Acad Sci USA 84:5374–5378Google Scholar
  15. Dewey RE, Siedow JN, Timothy DH, Levings CS III (1988) Science 239:293–295Google Scholar
  16. Dewey RE, Timothy DH, Levings CS III (1991) Curr Genet 20:475–482Google Scholar
  17. Fauron C, Havlik M (1989) Curr Genet 15:149–154Google Scholar
  18. Forde BG, Leaver CJ (1980) Proc Natl Acad Sci USA 77:418–422Google Scholar
  19. Forde BG, Oliver RJC, Leaver CJ (1978) Proc Natl Acad Sci USA 75:3841–3845Google Scholar
  20. Fox TD, Leaver CJ (1981) Cell 26:315–323Google Scholar
  21. Girbés T, Cabrer B, Modolell J (1979) Methods Enzymol 59:353–362Google Scholar
  22. Glab N, Wise RP, Pring DR, Jacq C, Slonimski P (1990) Mol Gen Genet 223:24–32Google Scholar
  23. Gray MW (1989) Annu Rev Cell Biol 5:25–50Google Scholar
  24. Gray MW, Hanic-Joyce PJ, Covello PS (1992) Annu Rev Plant Physiol Plant Mol Biol 43:145–175Google Scholar
  25. Gualberto JM, Lamattina L, Bonnard G, Weil J-H, Grienenberger J-M (1989) Nature 341:660–662Google Scholar
  26. Hack E, Leaver CJ (1983) EMBO J 2:1783–1789Google Scholar
  27. Hack E, Lin C, Yang H, Horner HT (1991) Plant Physiol 95:861–870Google Scholar
  28. Hanson MR (1991) Annu Rev Genet 25:461–486Google Scholar
  29. Hawkesford MJ, Liddell AD, Leaver CJ (1989) Plant Physiol 91:1535–1542Google Scholar
  30. Hiesel R, Wissinger B, Schuster W, Brennicke A (1989) Science 246:1632–1634Google Scholar
  31. Huang J, Lee S-H, Lin C, Medici R, Hack E, Myers AM (1990) EMBO J 9:339–347Google Scholar
  32. Isaac PG, Brennicke A, Dunbar SM, Leaver CJ (1985 a) Curr Genet 10:321–328Google Scholar
  33. Isaac PG, Jones VP, Leaver CJ (1985b) EMBO J 4:1617–1623Google Scholar
  34. Kenneli JC, Pring DR (1989) Mol Gen Genet 216:16–24Google Scholar
  35. Kozak M (1989 a) J Cell Biol 108:229–241Google Scholar
  36. Kozak M (1989 b) Mol Cell Biol 9:5073–5080Google Scholar
  37. Kozak M (1991) J Biol Chem 266:19867–19870Google Scholar
  38. Laemmli UK (1970) Nature 227:680–685Google Scholar
  39. Leaver CJ, Forde BG (1980) Mitochondrial genome expression in higher plants. In: Leaver CJ (ed) Genome organization and expression in plants. Plenum Press, New York, pp 407–425Google Scholar
  40. Leaver CJ, Hack E, Forde BG (1983) Methods Enzymol 97:476–484Google Scholar
  41. Levings CS III, Siedow JN (1992) Plant Mol Biol 19:135–147Google Scholar
  42. Logemann J, Schell J, Willmitzer L (1987) Anal Biochem 163:16–20Google Scholar
  43. Lonsdale DM, Leaver CJ (1988) Plant Mol Biol Rep 6(2):14–21Google Scholar
  44. Lonsdale DM (1989) The plant mitochondrial genome. In: Marcus A (ed) The biochemistry of plants, vol 15: molecular biology. Academic Press, San Diego, pp 229–295Google Scholar
  45. MacDonald RJ, Swift GH, Przybyla AE, Chirgwin JM (1987) Methods Enzymol 152:219–227Google Scholar
  46. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New YorkGoogle Scholar
  47. Newton KJ (1988) Annu Rev Plant Physiol Plant Mol Biol 39:503–532Google Scholar
  48. Newton KJ, Walbot V (1985) Proc Natl Acad Sci USA 82:6879–6883Google Scholar
  49. Pelham HRB, Jackson RJ (1976) Eur J Biochem 67:247–256Google Scholar
  50. Pring DR, Lonsdale DM (1989) Annu Rev Phytopathol 27:483–502Google Scholar
  51. Reisfeld A, Edelman M (1982) Translation of chloroplast poly A mRNA in the wheat germ system. In: Edelman M et al. (eds) Methods in chloroplast molecular biology. Elsevier, Amsterdam, pp 605–614Google Scholar
  52. Rochaix J-D, Malnoë P (1982) Use of DNA-RNA hybridizations for locating chloroplast genes and for estimating the size and abundance of chloroplast DNA transcripts. In: Edelman M et al. (eds) Methods in chloroplast molecular biology. Elsevier, Amsterdam, pp 477–490Google Scholar
  53. Tzagoloff A (1982) Mitochondria. Plenum Press, New YorkGoogle Scholar
  54. Ward GC, Levings CS III (1991) Plant Mol Biol 17:1083–1088Google Scholar
  55. Wise RP, Fliss AE, Pring DR, Gengenbach BG (1987) Plant Mol Biol 9:121–126Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Ethan Hack
    • 1
  • Carol A. Hendrick
    • 1
  • Salah M. Al-Janabi
    • 1
  • Virginia C. Crane
    • 1
  • Lois E. Girton
    • 1
  1. 1.Department of BotanyIowa State UniversityAmesUSA

Personalised recommendations