Summary
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1.
After a brief survey of the basic affinity electrophoresis concepts, the usual ways for preparing affinity electrophoresis ligands are examined.
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2.
Then results obtained on cholinesterases are reviewed. This section includes (a) structural and functional investigations on anionic sites, i.e., study of ligand-induced conformational change, organophosphate-induced “aging,” genetic variants, and active-site topology; and (b) characterization of cholinesterase conjugates (hybrid proteins) and glycoinositol phospholipid-anchored cholinesterases.
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3.
The future prospects of affinity electrophoresis, e.g., investigations on the esteratic site and exploration of the carbohydrate moiety, are emphasized in the concluding section.
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Masson, P. Structural and functional investigations of cholinesterases by means of affinity electrophoresis. Cell Mol Neurobiol 11, 173–189 (1991). https://doi.org/10.1007/BF00712808
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DOI: https://doi.org/10.1007/BF00712808